The new landscape of differentially expression proteins in placenta tissues of gestational diabetes based on iTRAQ proteomics

ABSTRACT: Proteomics screening of the DEPs of the normal and GDM maternal placentas was conducted by using isobaric tags for relative and absolute quantitation (iTRAQ). The bioinformatics analysis was performed on DEPs, and parallel reaction monitoring (PRM) was used to verify the DEPs. Sixty-eight DEPs in GDM placenta were identified by iTRAQ proteomics experiment; 21 of these DEPs were up-regulated, and 47 were down-regulated. The bioinformatics analysis showed that the regulation of transport, catabolic process of non-coding RNA (ncRNA), cytoskeleton and cell binding were the most abundant gene ontology terms, and RNA degradation was an important pathway for significant enrichment. Protein–protein interaction (PPI) network analysis showed that heterogeneous nuclear ribonucleoproteins A2/B1 (HNRNPA2B1), heterogeneous nuclear ribonucleoprotein A/B (HNRNPAB), heterogeneous nuclear ribonucleoprotein L (HNRNPL) and heterogeneous nuclear ribonucleoprotein A3 (HNRNPA3) were the core of up-regulated proteins. Band 3 anion transport protein (SLC4A1), spectrin beta chain erythrocytic (SPTB), ankyrin-1 (ANK1), spectrin beta chain non-erythrocytic 2 (SPTBN2), D-3-phosphoglycerate dehydrogenase (PHGDH) and exosome complex component RRP42 (EXOSC7) were the core of down-regulated proteins. These proteins were involved in the binding, splicing, processing, transport and degradation of RNA, as well as in the formation and maintenance of the cytoskeleton. PRM verification results showed that seven proteins, namely, epiplakin (EPPK1), cold-inducible RNA-binding protein (CIRBP), HNRNPA2B1, HNRNPAB, HNRNPL, Ras-related protein Rab-21 (RAB21) and Ras-related protein Rab-3B (RAB3B), were up-regulated, whereas SPTB and SLC4A1 were down-regulated.

ORGANISM(S): Homo Sapiens

SUBMITTER: Li Ge  

PROVIDER: PXD033280 | iProX | Sun Apr 17 00:00:00 GMT+01:00 2022

REPOSITORIES: iProX