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Nmyc co-IP and post-translational modification identification

ABSTRACT: Endogenous and exogenous N-Myc co-IP were performed in neuroblastoma cell lines and 293T cells. The co-IP products were pretreated and subjected to LC-MS/MS analysis.

ORGANISM(S): Homo Sapiens

SUBMITTER: Zhixiang Wu

PROVIDER: PXD033457 | iProX | Sun Oct 09 00:00:00 BST 2022

REPOSITORIES: iProX

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P300 Interacted With N-Myc and Regulated Its Protein Stability via Altering Its Post-Translational Modifications in Neuroblastoma.

Cheng Cheng C He Tian T Chen Kai K Cai Yuanxia Y Gu Yaoyao Y Pan Lijia L Duan Peiwen P Wu Yeming Y Wu Zhixiang Z

Molecular & cellular proteomics : MCP 20230126 3

MYCN amplification is an independent risk factor for poor prognosis in neuroblastoma (NB), but its protein product cannot be directly targeted because of protein structure. Thus, this study aimed to explore novel ways to indirectly target N-Myc by regulating its post-translational modifications (PTMs) and therefore protein stability. N-Myc coimmunoprecipitation combined with HPLC-MS/MS identified 16 PTM residues and 114 potential N-Myc-interacting proteins. Notably, both acetylation and ubiquiti ...[more]

PMID: 36708875

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