Dataset Information

Effects of corosolic acid on protein expression of in Bel-7402 and Bel-7404 cells

ABSTRACT: We treated Bel-7402 and Bel-7404 cells with corosolic acid, and then used TMT to analyze protein expression in both cell lines after treatment with corosolic acid

ORGANISM(S): Homo Sapiens

SUBMITTER: Yanfeng Xu

PROVIDER: PXD033462 | iProX | Wed Apr 27 00:00:00 BST 2022

REPOSITORIES: iProX

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Publications

Corosolic acid sensitizes ferroptosis by upregulating HERPUD1 in liver cancer cells.

Peng Yingxiu Y Li Ning N Tang Feifeng F Qian Chunmei C Jia Tingting T Liu Jingjin J Xu Yanfeng Y

Cell death discovery 20220829 1

Primary liver cancer is the third leading cause of cancer death in the world, and the lack of effective treatments is the main reason for the high mortality. Corosolic acid (CA) has been proved to have antitumor activity. In this study, we found that CA can sensitize liver cancer cells to ferroptosis, which is a regulated form of cell death characterized by iron-dependent lipid peroxides reaching lethal levels. Here, we revealed that CA can inhibit glutathione (GSH) synthesis via HERPUD1, decrea ...[more]

PMID: 36038536

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Project description:The fertility of dairy cows is challenged during early lactation and better nutritional strategies need to be developed to address this issue. Combined supplementation of folic acid and vitamin B12 improves energy metabolism in the dairy cow during early lactation. Therefore, the present study was undertaken to explore the effects of this supplement on gene expression in granulosa cells from the dominant follicle during the postpartum period. Multiparous Holstein cows received weekly intramuscular injection of 320 mg folic acid and 10 mg vitamin B12 (treated group) beginning 24 (SD 4) d before calving until 56 d after calving, whereas the control group received saline. The urea plasma concentration was significantly decreased during the pre-calving period, and the concentration of both folate and vitamin B12 were increased in treated animals. Milk production and dry matter intake were not significantly different between the two groups. Plasma concentrations of folates and vitamin B12 were increased in vitamin-treated animals. Daily dry matter intake was not significantly different between the 2 groups before (13.5 kg SE 0.5) and after (23.6 kg SE 0.9) calving. Average energy-corrected milk tended to be greater in vitamin-treated cows, 39.7 (SE 1.4) and 38.1 (SE 1.3) kg/d for treated and control cows, respectively. After calving, average plasma concentration of BHBA tended to be lower in cows injected with the vitamin supplement, 0.47 (SE 0.04) vs. 0.55 (SE 0.03) for treated and control cows, respectively. The ovarian follicle ? 12 mm in diameter was collected by ovarian pick-up after estrus synchronization. Recovered follicular fluid volumes were greater in the vitamin-treated group. A microarray platform was used to investigate the impact of treatment on gene expression of granulosa cells. Lower expression of genes involved in the cell cycle and higher expression of genes associated with granulosa cell differentiation prior to ovulation were observed. Selected candidate genes were analyzed by reverse transcription quantitative polymerase chain reaction. Although the effects of intramuscular injections of folic acid and vitamin B12 on lactational performance and metabolic status of animals were limited, Ingenuity Pathway Analysis of gene expression in granulosa cells suggests a stimulation of cell differentiation in vitamin-treated cows, which may be the result of an increase in LH secretion.

Dataset Information

Effects of corosolic acid on protein expression of in Bel-7402 and Bel-7404 cells

Publications

Corosolic acid sensitizes ferroptosis by upregulating HERPUD1 in liver cancer cells.

Similar Datasets

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