Transcriptomic and Proteomic analyses provide insights into the adaptive responses to the combined impact of salinity and alkalinity in Gymnocypris przewalskii
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ABSTRACT: In this study, the DIA/SWATH method was used to identify differentially expressed proteins (DEPs) under saline, alkaline, and saline-alkaline stresses. Combined analyses of the proteome and transcriptome, pairwise differential abundance analysis, and gene regulated networks were implemented to identify key responsive GO items and KEGG pathways. Furthermore, proteins and cellular pathways related to the alkaline-saline effect were characterized, and some were validated through Western blotting. Finally, the relationship of stresses with homeostasis processes, immune responses, and reproductive processes is discussed. These results established a proteomics database of Gymnocypris przewalskii and should be helpful for understanding the physiological regulation mechanisms under salinity, alkalinity, and salinity-alkalinity interactive stresses.
ORGANISM(S): Gymnocypris Przewalskii
SUBMITTER: Jian Liang
PROVIDER: PXD033567 | iProX | Sat Apr 30 00:00:00 BST 2022
REPOSITORIES: iProX
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