Dataset Information

Edwardsiella tarda strain EIB 202 lysine acetylation and succinylation

ABSTRACT: The antibiotics resistance of Edwardsiella tarda is prevailing, seeking a novel antimicrobial strategies extremely urgent. Lysine acylation has been proved to play important role on the bacterial physiological function while its role on bacterial antibiotics resistance mechanism still largely unclear. In this study, we investigated the lysine acetylation and succinylation profiles of E. tarda stain EIB202 using affinity antibody purification combined with LC MS/MS. A total of 1511 lysine-acetylation sites were identified on 589 proteins, and 2346 lysine-succinylation sites were further identified on 692 proteins of this pathogen. Further bioinformatics analysis showed that both PTMs prefer to enrich in TCA cycle, pyruvate metabolism. biosynthesis, and carbon metabolism. Besides, 948 peptides of 437 proteins were found to be overlap and associated with multiple metabolic pathways. Moreover, both the acetylation and succinylation were found in many antimicrobial resistance (AMR) proteins, suggesting its potentially vital role in antibiotic resistance. In general, our work provides insights into the acetylome and succinylome features responsible for the antibiotic resistance mechanism of E. tarda, which may facilitate future investigations on the pathogenesis of this bacterium.

ORGANISM(S): Edwardsiella Tarda Eib202

SUBMITTER: Xiangmin Lin

PROVIDER: PXD033818 | iProX | Thu May 12 00:00:00 BST 2022

REPOSITORIES: iProX

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Publications

Acetylome and Succinylome Profiling of Edwardsiella tarda Reveals Key Roles of Both Lysine Acylations in Bacterial Antibiotic Resistance.

Fu Yuying Y Zhang Lishan L Song Huanhuan H Liao Junyan J Lin Li L Jiang Wenjia W Wu Xiaoyun X Wang Guibin G

Antibiotics (Basel, Switzerland) 20220623 7

The antibiotic resistance of Edwardsiella tarda is becoming increasingly prevalent, and thus novel antimicrobial strategies are being sought. Lysine acylation has been demonstrated to play an important role in bacterial physiological functions, while its role in bacterial antibiotic resistance remains largely unclear. In this study, we investigated the lysine acetylation and succinylation profiles of E. tarda strain EIB202 using affinity antibody purification combined with LC-MS/MS ...[more]

PMID: 35884095

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Project description:The study of antimicrobial resistance (AMR) in infectious diarrhea has generally been limited to cultivation, antimicrobial susceptibility testing and targeted PCR assays. When individual strains of significance are identified, whole genome shotgun (WGS) sequencing of important clones and clades is performed. Genes that encode resistance to antibiotics have been detected in environmental, insect, human and animal metagenomes and are known as "resistomes". While metagenomic datasets have been mined to characterize the healthy human gut resistome in the Human Microbiome Project and MetaHIT and in a Yanomani Amerindian cohort, directed metagenomic sequencing has not been used to examine the epidemiology of AMR. Especially in developing countries where sanitation is poor, diarrhea and enteric pathogens likely serve to disseminate antibiotic resistance elements of clinical significance. Unregulated use of antibiotics further exacerbates the problem by selection for acquisition of resistance. This is exemplified by recent reports of multiple antibiotic resistance in Shigella strains in India, in Escherichia coli in India and Pakistan, and in nontyphoidal Salmonella (NTS) in South-East Asia. We propose to use deep metagenomic sequencing and genome level assembly to study the epidemiology of AMR in stools of children suffering from diarrhea. Here the epidemiology component will be surveillance and analysis of the microbial composition (to the bacterial species/strain level where possible) and its constituent antimicrobial resistance genetic elements (such as plasmids, integrons, transposons and other mobile genetic elements, or MGEs) in samples from a cohort where diarrhea is prevalent and antibiotic exposure is endemic. The goal will be to assess whether consortia of specific mobile antimicrobial resistance elements associate with species/strains and whether their presence is enhanced or amplified in diarrheal microbiomes and in the presence of antibiotic exposure. This work could potentially identify clonal complexes of organisms and MGEs with enhanced resistance and the potential to transfer this resistance to other enteric pathogens. We have performed WGS, metagenomic assembly and gene/protein mapping to examine and characterize the types of AMR genes and transfer elements (transposons, integrons, bacteriophage, plasmids) and their distribution in bacterial species and strains assembled from DNA isolated from diarrheal and non-diarrheal stools. The samples were acquired from a cohort of pediatric patients and controls from Colombia, South America where antibiotic use is prevalent. As a control, the distribution and abundance of AMR genes can be compared to published studies where resistome gene lists from healthy cohort sequences were compiled. Our approach is more epidemiologic in nature, as we plan to identify and catalogue antimicrobial elements on MGEs capable of spread through a local population and further we will, where possible, link mobile antimicrobial resistance elements with specific strains within the population.

Dataset Information

Edwardsiella tarda strain EIB 202 lysine acetylation and succinylation

Publications

Acetylome and Succinylome Profiling of <i>Edwardsiella tarda</i> Reveals Key Roles of Both Lysine Acylations in Bacterial Antibiotic Resistance.

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