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Subcellular Interactomes Revealed by Merging APEX with Cross-linking Mass Spectrometry

ABSTRACT: An integrating APEX proximity labeling and chemical cross-linking coupled with mass spectrometry (CXMS) platform named APEX-CXMS for spatially resolved subcellular interactome profiling in a high-throughput ma

ORGANISM(S): Homo Sapiens

SUBMITTER: Xiaoguang Lei

PROVIDER: PXD033838 | iProX | Thu Jul 21 00:00:00 BST 2022

REPOSITORIES: iProX

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Subcellular Interactomes Revealed by Merging APEX with Cross-Linking Mass Spectrometry.

Sun Mengze M Yuan Feng F Tang Yuliang Y Zou Peng P Lei Xiaoguang X

Analytical chemistry 20221020 43

Subcellular protein-protein interactions (PPIs) are essential to understanding the mechanism of diverse cellular signaling events and the pathogenesis of diseases. Herein, we report an integrated APEX proximity labeling and chemical cross-linking coupled with mass spectrometry (CXMS) platform named APEX-CXMS for spatially resolved subcellular interactome profiling in a high-throughput manner. APEX proximity labeling rapidly captures subcellular proteomes, and the highly reactive chemical cross-l ...[more]

PMID: 36265550

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