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Rat hippocampal neurons' proteomes

ABSTRACT: We induced over-expression and under-expression of Camk2b in cultured rat hippocampal neurons through transfection with lentivirus plasmids. Then isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomics followed by bioinformatics analyses were carried out to explore the impacts of Camk2b dysexpression on the proteome of the neurons.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Jian Zhou

PROVIDER: PXD033931 | iProX | Wed Jul 27 00:00:00 BST 2022

REPOSITORIES: iProX

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Effects of Camk2b Overexpression and Underexpression on the Proteome of Rat Hippocampal Neurons.

Yang Chen C Zhang Mingming M Li Shuiming S Yi Faping F Huang Haojun H Xie Hong H Liu Hangfei H Huang Rongzhong R Zhou Jian J

Neuroscience 20220827

Recent studies have demonstrated that Camk2b expression is modified in neuropsychiatric illnesses and potentially affects synaptic plasticity. However, the molecular events arising from Camk2b dysregulation are not fully elucidated and need to be comprehensively explored. In the present study, we first induced over-expression and under-expression of Camk2b in cultured rat hippocampal neurons through transfection with lentivirus plasmids. Then isobaric tag for relative and absolute quantitation ( ...[more]

PMID: 36041587

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