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Comprehensive evaluation of different TiO2-based phosphopeptide enrichment and fractionation methods for phosphoproteomics

ABSTRACT: We performed a comprehensive evaluation of four different TiO2-based phosphopeptide enrichment methods using different non-phosphopeptide excluders. Then, two phosphopeptide fractionation methods, including the ammonia-based and the TEA based high pH reversed-phase fractionation, are also evaluated.

ORGANISM(S): Homo Sapiens

SUBMITTER: Fuquan Yang

PROVIDER: PXD034264 | iProX | Thu Jun 02 00:00:00 BST 2022

REPOSITORIES: iProX

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Comprehensive Evaluation of Different TiO2-Based Phosphopeptide Enrichment and Fractionation Methods for Phosphoproteomics.

Li Jiaran J Wang Jifeng J Yan Yumeng Y Li Na N Qing Xiaoqing X Tuerxun Ailikemu A Guo Xiaojing X Chen Xiulan X Yang Fuquan F

Cells 20220628 13

Protein phosphorylation is an essential post-translational modification that regulates multiple cellular processes. Due to their low stoichiometry and ionization efficiency, it is critical to efficiently enrich phosphopeptides for phosphoproteomics. Several phosphopeptide enrichment methods have been reported; however, few studies have comprehensively compared different TiO2-based phosphopeptide enrichment methods using complex proteomic samples. Here, we compared four TiO2 ...[more]

PMID: 35805136

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