Dataset Information

Comparative proteomic analysis of two divergent Lingzhi strains

ABSTRACT: It is reported that heat stress could induce the biosynthesis of ganoderic acids and enhance the production of G. lingzhi polysaccharides. However, heat stress inhibits mycelium growth and reduces hyphal branching, resulting in a significant reduction in mycelial dry weight. Tandem mass tag-based (TMT) comparative proteomic analysis was performed to explore the potential mechanisms associated with thermotolerance in G. lingzhi in this study.

ORGANISM(S): Ganoderma Lingzhi

SUBMITTER: Huiping Hu

PROVIDER: PXD034524 | iProX | Wed Jun 15 00:00:00 GMT+01:00 2022

REPOSITORIES: iProX

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Comparative proteomic analysis of two divergent strains provides insights into thermotolerance mechanisms of Ganoderma lingzhi.

Cai Manjun M Wu Xiaoxian X Liang Xiaowei X Hu Huiping H Liu Yuanchao Y Yong Tianqiao T Li Xiangmin X Xiao Chun C Gao Xiong X Chen Shaodan S Xie Yizhen Y Wu Qingping Q

Fungal genetics and biology : FG & B 20230504

Heat stress (HS) is a major abiotic factor influencing fungal growth and metabolism. However, the genetic basis of thermotolerance in Ganoderma lingzhi (G. lingzhi) remains largely unknown. In this study, we investigated the thermotolerance capacities of 21 G. lingzhi strains and screened the thermo-tolerant (S566) and heat-sensitive (Z381) strains. The mycelia of S566 and Z381 were collected and subjected to a tandem mass tag (TMT)-based proteome assay. We identified 1493 differentially express ...[more]

PMID: 37146899

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Project description:Cellular responses to maladaptive environmental changes—stresses—allow for organismal adaptation to diverse and dynamic conditions. Across the tree of life, cells upregulate a highly conserved transcriptional program in response to so-called proteotoxic stresses such as heat shock. Correspondingly, in eukaryotes, these stresses induce the formation of biomolecular condensates, clusters of mRNA and protein which are referred to as stress granules under severe stress. However, major questions remain about this stress-induced response. How conserved is the condensation response relative to the transcriptional response? How does it vary across environmental niches, and to what extent does it correspond with the conserved transcriptional response? To answer these fundamental questions, we studied the growth, transcriptional, and condensation heat-induced stress responses in three fungal species adapted to thrive in different thermal environments: cryophilic S. kudriavzevii, mesophilic S. cerevisiae, and thermotolerant K. marxianus. Here we show that transcriptional heat shock responses track each species’ evolved temperature range of growth. Further, orthologous proteins—including poly(A)-binding protein, Pab1, a core marker of stress granules—form condensates in vivo at temperatures systematically tuned to the temperature at which the organisms activate the transcriptional heat shock response and slow their growth. In vitro, purified Pab1 from each species condenses autonomously at niche-specific temperatures. Homologous mutations in Pab1 cause similar shifts in relative condensation temperature across species, and crucially, mutations which suppress condensation in vitro also reduce fitness during heat stress. Our findings indicate that stress-induced protein condensation is adaptive, conserved, integrated with the growth and transcriptional responses, and tuned to features of the cellular and organismal environment to initiate at niche-specific levels.

Dataset Information

Comparative proteomic analysis of two divergent Lingzhi strains

Publications

Comparative proteomic analysis of two divergent strains provides insights into thermotolerance mechanisms of Ganoderma lingzhi.

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