The antibacterial mechanism of sertindole against Staphylococcus aureus

ABSTRACT: Clinically isolated MRSA strain was inoculated and cultured to exponential growth phase (OD600 at 0.5) in TSB and divided into two groups, the control group and the drug treated group. Sertindole was added to the drug treated group until a final concentration of 1/2× MIC reached, and DMSO added as Lomitapide in the control group. Then, cultures in both groups were cultivated for 2h at 180rpm. The microbial was centrifuged at 12000rpm for 10min, washed twice with Phosphate Buffer Saline (PBS). Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer.

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Zewen Wen  

PROVIDER: PXD035364 | iProX | Thu Jul 14 00:00:00 BST 2022

REPOSITORIES: iProX