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PBC, an easy and efficient strategy for high-throughput protein C-terminome profiling

ABSTRACT: So far, high-efficiency and high-throughput analysis of C-terminal peptides is still full of challenges. In order to make the chemical derivatization-based strategies more practical and efficient, in this study we developed a new method, named 2-pyridinecarboxaldehyde (2PCA) and biotin labeling based C-terminomics (PBC), for high-throughput and highly efficient enrichment of C-terminal peptides.

ORGANISM(S): Homo Sapiens

SUBMITTER: Minjia Tan

PROVIDER: PXD035645 | iProX | Mon Aug 01 00:00:00 BST 2022

REPOSITORIES: iProX

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PBC, an easy and efficient strategy for high-throughput protein C-terminome profiling.

Zhai Linhui L Wang Le L Hu Hao H Liu Quan Q Lee Sangkyu S Tan Minjia M Zhang Yinan Y

Frontiers in cell and developmental biology 20220831

High-throughput profiling of protein C-termini is still a challenging task. Proteomics provides a powerful technology for systematic and high-throughput study of protein C-termini. Various C-terminal peptide enrichment strategies based on chemical derivatization and chromatography separation have been reported. However, they are still costly and time-consuming, with low enrichment efficiency for C-terminal peptides. In this study, by taking advantage of the high reaction selectivity of 2-pyridin ...[more]

PMID: 36120566

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