Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

2 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The proteome and phosphorylproteome of human early erythropoiesis

ABSTRACT: In order to revealed the multiple protein functional modules and kinases networks of human early erythropoiesis. We isolated the CD34+ cells drived from Human umbilical cord blood samples and induced to undergo erythropoiesis in vitro. Then, the cultured erythroid cells were sorted with FACS and sampled for proteome and phosphoproteome analysis.

ORGANISM(S): Homo Sapiens

SUBMITTER: Jing Liu

PROVIDER: PXD035785 | iProX | Wed Aug 03 00:00:00 BST 2022

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Publications

Comprehensive proteomic analysis reveals dynamic phospho-profiling in human early erythropoiesis.

Peng Yuanliang Y Tang Li L Li Yanan Y Song Jianhui J Liu Hong H Wang Pan P Zhong Zhizhou Z Yang Yifei Y Wang Shihui S Chen Lixiang L Zhang Ji J Zhang Shijie S Wang Zi Z Li Min M Liang Long L Liu Jing J

British journal of haematology 20220816 3

Normal early erythropoiesis depends on the precise regulation of protein expression and phosphorylation modification. Dysregulation of protein levels or modification contributes to erythroid disorders. To date, the dynamics of protein phosphorylation profiling across human erythroid development is not fully understood. Here, we characterized quantitative proteomic and phosphoproteomic profiling by tandem mass-tagging technology. We systemically built phospho-expression profiling and expression c ...[more]

PMID: 35974424

Similar Datasets

A clinical study of cord blood-derived NK cells against recurrence and metastasis in patients with stage IIC and IIIC colorectal cancer

Project description:Interventions: Test group:Postoperative adjuvant chemotherapy plus infusion of NK cells derived from human umbilical cord blood;control group:Postoperative adjuvant chemotherapy Primary outcome(s): Recurrence and metastasis rates Study Design: Parallel

| 2725823 | ecrin-mdr-crc

Proteomic analysis of exosomes isolated from human umbilical cord serum by mass spectrometry and preliminary study of their biological activities in cultured hepatocellular carcinoma cell lines

Project description:Exosomes are membranous extracellular vesicles 50–100 nm in size and are involved in cellular communication via the delivery of proteins, lipids, and RNAs. Emerging evidence shows that exosomes play a critical role in cancer. A recent study has revealed that maternal and umbilical cord serum-derived exosomes may enhance endothelial cell proliferation and migration. However, the role of exosomes isolated from the human umbilical cord in cancer development has not been investigated. To explore the potential differences in the composition and function of proteins from umbilical cord blood exosomes and maternal serum exosomes, we conducted a proteomic analysis of exosomes by mass spectrometry and bioinformatics analysis. We used the CCK-8 assay and flow cytometry to study the biological effects of umbilical serum exosomes on hepatoma cells. Our study shows that umbilical cord blood is enriched with proteins involved in ECM-receptor interactions, which may be closely related to cell metastasis and proliferation. Our findings indicate that exosomes derived from human umbilical serum can suppress the viability of hepatoma cells and may induce apoptosis of hepatoma cells. This evidence suggests that umbilical cord serum-derived exosomes may be potential leads for the development of biotherapy for liver cancer.

2022-02-17 | PXD025079 | Pride

Quantitative shotgun proteomics (TMT) of EVs in human MSCs

Project description:Quantitative shotgun proteomic analysis (TMT) of the effect of inhibition of MIR21 in the EV protein cargo of human, Umbilical cord-derived Mesenchymal Stem Cells.

2021-02-27 | MSV000086963 | MassIVE

Proteomic profiling of human mesenchymal stem cells derived from umbilical cord or adipose tissue

Project description:We performed a proteomic profiling of human mesenchymal stem cells (hMSCs) derived from adipose tissue or umbilical cord.

2018-07-11 | PXD009893 | Pride

Gene Expression Profile of Human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) Culutured in Chemical Defined Serum Free Medium

Project description:Human umbilical cord mesenchymal stem cells maintained multipotency and immunosuppressive ability when being cultured in chemical defined serum free medium, but gained different gene expression profile. We used microarrays to identify the transcriptional difference between human umbilical cord mesenchymal stem cells cultured in serum containing medium and chemical defined serum free medium. human umbilical cord mesenchymal stem cells were cultured in conventional serum containing medium and chemical defined serum free medium separately. Total RNA was extracted and hybridized on Affymetrix microarrays.

2014-10-28 | E-GEOD-62665 | biostudies-arrayexpress

Human umbilical cord blood-derived mesenchymal stem cells from 2 donors

Project description:screening of signature deterimes the individual variations in the therapeutic efficacy of human umbilical cord blood-derived mesenchymal stem cells There is paucity of information whether human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) from separate donors might have different effects on improving myocardial repair after myocardial infarction (MI).

2011-02-19 | GSE27414 | GEO

Gene Expression Profile of Human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) Culutured in Chemical Defined Serum Free Medium

2014-10-28 | GSE62665 | GEO

Gene regulatory networks underlying intrinsic properties in human hematopoietic stem cell ontogeny

Project description:Human hematopoiesis starts at early yolk sac and undergoes site- and stage-specific changes over development. However, the gene regulatory networks (GRN) that drive the developmental changes in naive hematopoietic stem/progenitor cells (HSPCs) remain poorly understood. Here, we analyzed single-cell transcriptome of human naive HSPCs in different developmental stages, including yolk-sac (YS), AGM, fetal liver (FL), umbilical cord blood (UCB) and adult peripheral blood (PB) mobilized HSPCs. These stage-specific HSPCs display differential intrinsic properties, such as metabolism, self-renewal, differentiating potentialities etc. We generated highly co-related GRN modules underlying the differential HSC key properties. Particularly, we identified GRNs and key regulators controlling lymphoid potentiality, self-renewal and aerobic respiration in human HSCs. Introducing selected regulators promotes establishing key HSC functions in HSPCs derived from human pluripotent stem cells. Therefore, GRNs underlying key intrinsic properties of human HSCs provide a valuable guide to generate fully functional HSCs in vitro.

2024-03-19 | GSE224972 | GEO

CBCD34-TGFbeta targets

Project description:TGFbeta regulated genes in human umbilical cord blood CD34+ cells Keywords: other

2004-10-28 | GSE1802 | GEO

Transcription profiling of human circulating blood reticulocytes from adult and cord blood to provide a robust description of genes transcribed at the final stages of erythroblast maturation

Project description:RNA from circulating blood reticulocytes was utilized to provide a robust description of genes transcribed at the final stages of erythroblast maturation. After depletion of leukocytes and platelets, Affymetrix HG-U133 arrays were hybridized with probe from total RNA isolated from blood sampled from 14 umbilical cords and 14 healthy adult humans. Experiment Overall Design: Peripheral blood from 14 normal adult and 14 human umbilical cord were purified to obtain packed red blood cells and their purity was assessed by CELL-DYN4000. The contaminated WBC count was less than 1 out of 1 million cells. Total RNA from purified reticulocytes included in the purified red blood cells was extracted, labeled, and hybridized onto Affymetrix HG-U133 A and B arrays.

2007-11-01 | E-GEOD-6236 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data