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Effects of 3D printing HUVEC(vefg165+) -laden hydrogel on diabetic wound healing

ABSTRACT: By constructing specific lentiviral vector, vefg165 protein was overexpressed in HUVEC cells. Using light-curing bioprinting, HUVEC(vefg165+)-loaded hydrogels and HUVEC(vector)-loaded hydrogels were prepared, and were used to treat rat diabetic wounds. The regenerated skin tissues (five samples for each group) were analyzed with high-throughput proteomics.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: JIanan Ren

PROVIDER: PXD036045 | iProX | Fri Aug 12 00:00:00 BST 2022

REPOSITORIES: iProX

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A click chemistry-mediated all-peptide cell printing hydrogel platform for diabetic wound healing.

Huang Jinjian J Yang Rong R Jiao Jiao J Li Ze Z Wang Penghui P Liu Ye Y Li Sicheng S Chen Canwen C Li Zongan Z Qu Guiwen G Chen Kang K Wu Xiuwen X Chi Bo B Ren Jianan J

Nature communications 20231129 1

High glucose-induced vascular endothelial injury is a major pathological factor involved in non-healing diabetic wounds. To interrupt this pathological process, we design an all-peptide printable hydrogel platform based on highly efficient and precise one-step click chemistry of thiolated γ-polyglutamic acid, glycidyl methacrylate-conjugated γ-polyglutamic acid, and thiolated arginine-glycine-aspartate sequences. Vascular endothelial growth factor 165-overexpressed human umbilical vein endotheli ...[more]

PMID: 38030636

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