Dataset Information
PUP-IT2 as an proximity labeling strategy
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens
SUBMITTER: 
Min Zhuang
PROVIDER: PXD036047 | iProX | Fri Aug 12 00:00:00 BST 2022
REPOSITORIES: iProX
Publications
PUP-IT2 as an alternative strategy for PUP-IT proximity labeling.
Frontiers in molecular biosciences 20220929
PUP-IT is a proximity labeling method based on the prokaryotic enzyme PafA. PafA mediates the ligation of Pup, a small peptide, to the proximal proteins. It is different from other proximity labeling methods, such as BioID and APEX, in that both the enzyme and the labeling tag are proteins, which allows for potential <i>in vivo</i> applications. All proximity labeling involves the genetic fusion of the proximity labeling enzyme with the bait protein. However, PafA is a 55 kDa enzyme which someti ...[more]
