Dataset Information

Proteomic study on lavage fluid of Dairy cows 30 days after delivery

ABSTRACT: Lipopolysaccharide (LPS) is a component of the outer membrane of gram-negative bacteria that is involved in pathogenic processes leading to postpartum metritis and endometritis in dairy cow. It also causes inflammation of the endometrium. This study aimed to investigate the effects of LPS on cow endometrial epithelial cells (BEECs). In this study, the label-free LC–MS/MS approach was employed to investigate the proteome profile of cow endometrial epithelial cells. A total of 1121 proteins were identified in the control group and model group, of which 186 differential proteins were accurately quantified. The proteomic results were verified by WB and IF techniques, and the same conclusion was obtained

ORGANISM(S): Bos Taurus

SUBMITTER: Yaping Jin

PROVIDER: PXD036248 | iProX | Tue Aug 23 00:00:00 BST 2022

REPOSITORIES: iProX

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Proteomic analysis of uterine lavage fluid of dairy cows at different time after delivery by mass spectrometry.

Jiang Xingcan X Li Ziyuan Z Chang Xiyu X Huang Cong C Qiu Rendong R Wang Aihua A Lin Pengfei P Tang Keqiong K Chen Huatao H Zhou Dong D Jin Yaping Y

Theriogenology 20230523

Endometritis is a common disease in the reproductive system, which is the infection and inflammation of the endometrium. In severe cases, it can affect the myometrium and adversely affect the subsequent fertility of dairy cows. We used a mass spectrometry-based technique to compare proteomics of uterine lavage fluid between healthy cows and cows with cytological endometritis classified according to 100-day postpartum pregnancy results and diagnosis result. The uterine lavage fluid of dairy cows ...[more]

PMID: 37257220

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Project description:Cesarean section scar diverticulum (CSD) has become a formidable obstacle preventing women receiving CS from reproducing. However, the pathogenesis of CSD remains unexplored. In this study, we characterized the cervical microbiota, metabolome and endometrial transcriptome of women with CSD. Based on the 16s rRNA results of cervical microbes, the microbial diversity in the CSD group was higher than that in the control group. Lactobacillus were significantly decreased in the CSD group and were mutually exclusive with potentially harmful species (Sphingomonas, Sediminbacterium and Ralstonia) abnormally elevated in CSD. The microbiota in the CSD group exhibited low activity in carbohydrate metabolism and high activity in fatty acid metabolism, as confrmed by the metabolomic data. The metabolomic characterization identified 6,130 metabolites, of which 34 were significantly different between the two groups. In the CSD group, N-(3-hydroxy-eicosanoid)-homoserine lactone and Ternatin were significantly increased, in addition to the marked decrease in fatty acids due to high consumption. N-(3-hydroxyeicosanoyl)-homoserine lactone is a regulator that promotes abnormal apoptosis in a variety of cells, including epithelial cells and vascular endothelial cells. This abnormal apoptosis of endometrial epithelial cells and neovascularization was also reflected in the transcriptome of the endometrium surrounding the CSD. In the endometrial transcriptome data, the upregulated genes in the CSD group were active in negatively regulating the proliferation of blood vessel endothelial cells, endothelial cells and epithelial cells. This alteration in the host’s endometrium is most likely influenced by the abnormal microbiota, which appears to be confirmed in the results by integrating host transcriptome and microbiome data. For the first time, this study described the abnormal activity characteristics of microbiota and the mechanism of host-microbiota interaction in CSD.

Dataset Information

Proteomic study on lavage fluid of Dairy cows 30 days after delivery

Publications

Proteomic analysis of uterine lavage fluid of dairy cows at different time after delivery by mass spectrometry.

Similar Datasets

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