Proteomics revealed the effects of high O2 stress on nucleic acid metabolism, yellowing and senescence in postharvest broccoli
Ontology highlight
ABSTRACT:
ORGANISM(S): Brassica Oleracea Var. Oleracea
SUBMITTER: 
Yanyin Guo
PROVIDER: PXD036422 | iProX | Wed Aug 31 00:00:00 BST 2022
REPOSITORIES: iProX
Similar Datasets
Project description:Senescence (or aging) is the final stage of plant development; senescence involves a complex programmed process that is closely related to biotic and abiotic stresses and is regulated by fine-tuned molecular mechanisms. Postharvest vegetative organs undergo a series of physiological and biochemical changes during senescence. However, if postharvest plant organs were not scientifically processed or cured, there would be a great loss in their commercial value during curing or storage. Thus, studying the molecular mechanisms underlying senescence of plant organs will enhance understanding of such a fundamental biological process and might provide new insights into controlling senescence and improving commercial value during curing or storage. However, few studies have focused specifically on the molecular mechanism of leaf senescence affecting tobacco quality at the proteomic level during the yellowing stage. The main objectives of this work were to determine the protein profile changes associated with different curing stages and to establish links between protein profile changes and senescence physiology to provide a more in-depth understanding of the mechanism of senescence in postharvest tobacco leaves under oxidation, heat/high temperature, dehydration and starvation stresses at the proteomic level. This study provides new insights into the postharvest physiology and molecular mechanism of senescence at the proteomic level in postharvest leaves of tobacco, a model solanaceous plant, in response to curing and senescence during the yellowing stage.
2020-01-06 | PXD016941 |
Project description:Both exogenously supplied and transgenic induced cytokinin production can effectively delay senescence of broccoli florets during postharvest storage. However, a substantial comparison between the mechanisms of these two treatments on delaying broccoli florets senescence was absent. Here, we conduct microarray analysis on broccoli florets of N6-benzylaminopurine treated and ipt-transgenic broccoli that harbor a senescence-associated-gene promoter triggering isopentenyltransferase gene expression during postharvest storage. Analysis used RNA of Green King inbred line 104 as control sample for comparison to the experimental samples of ipt-transgenic line 102, 103 and parental line Green King as well as 10 ppm BA treated Green King at harvest and after postharvest storage at 25 centigrade in the dark for 4 days.
2013-05-01 | E-GEOD-42609 | biostudies-arrayexpress
Project description:Nucleic Acid Sequence Submission
| PRJEB103858 | ENA
Project description:Here, we investigated the role of STN7 kinase of photosynthetic protein complexes in leaf senescence. Defects in STN7 accelerated leaf yellowing and decreased the efficiency of leaf photosynthetic complexes. To identify STN7 targets, we profiled global proteomes and phosphoproteomes of thylakoid membranes from wild type and STN7 mutant leaves during leaf senescence. Proteome data revealed senescence-associated alterations of protein complexes associated with photosynthesis, translation, lipid metabolism, and stress response during leaf senescence.
2025-12-08 | PXD033330 | Pride
Project description:Here, we investigated the role of STN7 kinase of photosynthetic protein complexes in leaf senescence. Defects in STN7 accelerated leaf yellowing and decreased the efficiency of leaf photosynthetic complexes. To identify STN7 targets, we profiled global proteomes and phosphoproteomes of thylakoid membranes from wild type and STN7 mutant leaves during leaf senescence. Proteome data revealed senescence-associated alterations of protein complexes associated with photosynthesis, translation, lipid metabolism, and stress response during leaf senescence.
2025-12-08 | PXD033333 | Pride
Project description:1. Comparison of two near isogenic lines between which the genome differs only for the region of the leaf-yellowing QTL Y3-4 on chromosome III.<br> Experiment description: <br><br> The lines HIF404-Sha and HIF404-Bay were grown on 3 mM nitrate in growth chamber according to Loudet et al . (2002, TAG 104:1173-84) until phenotypic yellowing symptoms differed between them. Plants were collected 49 days after sowing.<br><br> 2. Comparison of sugar effect on the leaf senescence progress using three RIL from the Bay-0 x shahdara population that exhibited differential leaf yellowing symptoms.<br> Experiment description: The recombinant inbred lines RIL310 (hypersenescing and early-senescing), RIL232 (early--senescing) et RIL045 (late-senescing) were grown in petri dishes (4,7 mM Nitrogen, with (LNG) or without (LN) glucose 2% until some difference of Fv/Fm trait can be observed (Wingler et al. 2004, New Phytol, 161 : 781-789). Growth experiments were performed in A. Wingler lab, University College of London.
2006-04-01 | E-MEXP-387 | biostudies-arrayexpress
Project description:Both exogenously supplied and transgenic induced cytokinin production can effectively delay senescence of broccoli florets during postharvest storage. However, a substantial comparison between the mechanisms of these two treatments on delaying broccoli florets senescence was absent. Here, we conduct microarray analysis on broccoli florets of N6-benzylaminopurine treated and ipt-transgenic broccoli that harbor a senescence-associated-gene promoter triggering isopentenyltransferase gene expression during postharvest storage.
2013-05-01 | GSE42609 | GEO
Project description:Leaf senescence is a developmental process designed for nutrient recycling and relocation to maximize growth competence and reproductive capacity of plants. Thus, plants integrate developmental and environmental signals to precisely control senescence. However, it remains largely elusive as to how plants coordinate genetic, epigenetic, and metabolic pathways for the regulation of senescence. To genetically dissect the complex regulatory mechanism underlying leaf senescence, we identified an early leaf senescence mutant, rse1. RSE1 encodes a putative glycosyltranferase. Loss-of-function mutations in RSE1 resulted in precocious leaf yellowing and up-regulation of senescence maker genes, indicating enhanced leaf senescence. Transcriptome analysis revealed that salicylic acid (SA) and defense signaling cascades were activated in rse1 prior to the onset of leaf senescence. In agreement with the phenotypes, we found that SA accumulation was significantly increased in rse1. We also discovered that the rse1 phenotypes are dependent on SA-INDUCTION DEFICIENT 2 (SID2), indicating a role of SA in accelerated leaf senescence in rse1. Furthermore, RSE1 protein was localized to the cell walls, and rse1 displayed increased glucose contents in the cell walls, implying a possible link between the cell walls and RSE1 function. Together, we show that RSE1 negatively modulate leaf senescence through an SID2-dependent SA signaling pathway.
2019-10-18 | GSE132740 | GEO
Project description:Leaf senescence can be triggered by jasmonic acid (JA) and darkness. There are scattered reports about JA and dark-induced senescence, respectively. While the precise regulatory mechanisms that integrate these two factors to initiate and regulate leaf senescence have not been identified. Here, we report a transcriptional regulating module centered on novel WRKY transcription factor that is responsible for both JA and dark-induced leaf senescence in tomato. The expression levels of SlWRKY37 together with the master transcription factor in JA signaling SlMYC2 could be significantly induced by both MeJA and dark treatments. SlMYC2 directly binds to the promoter of SlWRKY37 to active its expression. Knock out of SlWRKY37 inhibited JA and dark-induced leaf senescence. Transcriptome analysis revealed 1312 differentially expressed genes between slwrky37-CR and SlWRKY37-OE, including genes involved in JA synthesis as well as several senescence-associated genes (SAGs). We characterized SlWRKY53 and SlSGR1 as direct transcriptional targets of SlWRKY37 to regulate leaf senescence. Moreover, SlWRKY37 interacts with SlVQ7 protein in vivo and the interaction enhances its binding ability to the promoters and transcriptional activation to downstream target genes. In addition, SlWRKY37 is phosphorylated at the post-translational level. Phosphorylation of SlWRKY37 is essential for its protein interaction and transcriptional activation, indicating phosphorylation modification has a great effect on the function of SlWRKY37 protein. Our study reveals the physiological and molecular functions of SlWRKY37 in leaf senescence and offered a target gene to retard leaf yellowing by reducing the sensitivity to internal and external senescence signal such as JA and darkness.
2022-06-01 | GSE186072 | GEO
Project description:The postharvest senescence processes of citrus fruits were analyzed transcriptomic. The present study was aimed to: further uncover the rind-flesh communication of hesperidium; characterize the differential storage behaviors of different citrus varieties; reveal the important changes during storing process; and demonstrate the specific non-climacteric characteristics of citrus fruits.
2014-12-03 | GSE63706 | GEO