Dataset Information

Identification of the Key Protein Associated with Different Hair Types in Sheep and Goat

ABSTRACT: Animal-derived fiber has the characteristics of being light, soft, strong, elastic and a good thermal insulator and it is widely used in many industries and traditional products, so it plays an important role in the economy of some countries. Variations among phenotypes of wool fibers, among species and breeds, are important for industry. We found the mean fiber diameter of cashmere was significantly smaller than sheep wool ( P < 0.01 ), and sheep wool was significantly smaller than goat wool ( P < 0.01 ). Compared with the traditional proteomics technology, we analyzed cashmere, Guard hair, and wool by Laber-free proteomics technology and detected 159, 204, and 70 proteins respectively. Through the sequential windowed acquisition of all theoretical fragmentations (SWATH), 41 and 54 differentially expressed proteins were successfully detected in the three type fibers. Protein-protein interaction network analysis of differentially expressed proteins revealed many strong interactions related to KRT85、KRTAP15-1 and KRTAP3-1. The final analysis showed that the proportion of KRT85、KRTAP15-1 and KRTAP3-1 might be the key protein leading to the difference in fiber diameter and could be used as a potential molecular marker for distinguishing different fiber types.

ORGANISM(S): Ovis Aries Capra Hircus

SUBMITTER: Zhihong Liu

PROVIDER: PXD036685 | iProX | Fri Sep 09 00:00:00 BST 2022

REPOSITORIES: iProX

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Identification of the key proteins associated with different hair types in sheep and goats.

Zhang Chongyan C Qin Qing Q Liu Zhichen Z Xu Xiaolong X Lan Mingxi M Xie Yuchun Y Wang Zhixin Z Li Jinquan J Liu Zhihong Z

Frontiers in genetics 20220923

Animal-derived fiber has the characteristics of being light, soft, strong, elastic and a good thermal insulator, and it is widely used in many industries and traditional products, so it plays an important role in the economy of some countries. Variations in phenotypes of wool fibers among different species and breeds are important for industry. We found that the mean fiber diameter of cashmere was significantly smaller than that of sheep wool (<i>p</i> < 0.01), and sheep wool was significantly s ...[more]

PMID: 36212123

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Project description:Luchuan pig is a typical obese pig breed in China, and the diameter and area of its longissimus dorsi muscle fibers are significantly smaller than those of Duroc (lean) pig. Skeletal muscle fiber characteristics are related to meat quality of livestock. The smaller the diameter and area of muscle fiber, the better the meat quality. The diameter and area of muscle fibers are related to muscle growth and development. Therefore, we used the assay for transposase-accessible chromatin using sequencing (ATAC-seq) and RNA sequencing (RNA-seq) analysis to investigate the potential mechanism underlying the difference in skeletal muscle growth and development between the two types of pigs. First, transposase-accessible chromatin was analyzed to map the landscape of open chromatin regions and transcription factor binding sites. We identified several transcription factors that potentially affected muscle growth and development, including TFAP4, MAX, NHLH1, FRX5, and TGIF1. We also found that transcription factors with basic helix-loop-helix structures had a preference for binding to genes involved in muscle development. Then, by integrating ATAC-seq and RNA-seq, we found that the Wnt signaling pathway, the mTOR signaling pathway, and other classical pathways regulate skeletal muscle development. In addition, we found some pathways that might regulate skeletal muscle growth, such as parathyroid hormone synthesis, secretion, and action, synthesis and degradation of ketone bodies, and the thyroid hormone signaling pathway, which were significantly enriched. After further study, we identified a number of candidate genes (ASNS, CARNS1, G0S2, PPP1R14C, and SH3BP5) that might be associated with muscle development. We also found that the differential regulation of chromatin openness at the level of some genes was contrary to the differential regulation at the level of transcription, suggesting that transcription factors and transcriptional repressors may be involved in the regulation of gene expression. Our study provided an in-depth understanding of the mechanism behind the differences in muscle fibers from two species of pig and provided an important foundation for further research on improving the quality of pork.

Dataset Information

Identification of the Key Protein Associated with Different Hair Types in Sheep and Goat

Publications

Identification of the key proteins associated with different hair types in sheep and goats.

Similar Datasets

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