Proteomics

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Identification of ALK3-associated proteins


ABSTRACT: The epitope-tagging strategy to isolate ALK3-interacting protein complexes was performed. In brief, 293T cells were transfected with pcDNA3.1-FLAG-ALK3 for 48 hours. The cell pellets were resuspended in IP lysis buffer, and centrifuged at 12,000 rpm for 30 minutes at 4 °C. The cell lysates were filtered with 0.45 μm syringe filters, and then the supernatants were incubated with anti-FLAG antibody or normal IgG (negative control) overnight at 4 °C, and then incubated with Protein A/G agarose beads (sc-2003, Santa Cruz) for 6 hours at 4 °C. After that, the beads were washed with IP lysis buffer for 3 times and collected, and glycine-HCl solution (pH=3.5) was used to elute the bound polypeptides. The final eluates were subjected to an in-solution digestion and further mass spectrometry peptide sequencing.

ORGANISM(S): Homo Sapiens

SUBMITTER: Zeyu Cai  

PROVIDER: PXD037210 | iProX | Sat Oct 08 00:00:00 GMT+01:00 2022

REPOSITORIES: iProX

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