Dataset Information

Metabolomic interplay between the gut microbiome and plasma in CSA-AKI patients

ABSTRACT: Low cardiac output and kidney congestion are related to cardiac surgery-associated acute kidney injury (CSA-AKI), which is one of the most prevalent complications of cardiac surgery. This pathological process may be connected with a great risk of developing chronic kidney disease and mortality. This study aimed to investigate the possible correlations between gut microbiota metabolism and CSA-AKI.

ORGANISM(S): Homo Sapiens

SUBMITTER: Chunbo Chen

PROVIDER: PXD037304 | iProX | Tue Oct 11 00:00:00 BST 2022

REPOSITORIES: iProX

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Metabolomic interplay between gut microbiome and plasma metabolome in cardiac surgery-associated acute kidney injury.

Bai Yunpeng Y Huang Wendong W Jiang Xinyi X Xu Wang W Li Ying Y Wang Yirong Y Huang Sumei S Wu Kunyong K Hu Linhui L Chen Chunbo C

Rapid communications in mass spectrometry : RCM 20230601 11

<h4>Rationale</h4>Cardiac surgery-associated acute kidney injury (CSA-AKI) is a prevalent complication of cardiac surgery, which may be associated with a great risk of developing chronic kidney disease and mortality. This study aimed to investigate the possible links between gut microbiota metabolism and CSA-AKI.<h4>Methods</h4>A prospective cohort of patients who underwent cardiac surgery was continuously recruited, who were further divided into CSA-AKI group and Non-AKI group based on clinical ...[more]

PMID: 36918294

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Project description:Objectives: Secretion of extracellular vesicles (EV) and associated micro-RNAs (miR) is altered during cellular stress and may serve as biomarkers of organ injury. We hypothesized that measuring changes in urinary levels of EV and miR will predict the onset of acute kidney injury in cardiac surgery patients. Design: Predictive accuracy biomarker study performed in the cohort of the REVAKI-2 trial Setting: Single center ICU between September 2015 and September 2018 Interventions: Intravenous sildenafil citrate 12.5 mg kg-1 over 150 min or dextrose 5% at the commencement of surgery. Measurements and main results: Urine samples were collected before and 24 hours after the procedure from 93 cardiac surgery patients. Urine EV concentrations and size distribution were assessed using NanoSight. EV derivation and levels were measured using flow cytometry. Samples from 10 selected patients were sequenced to detect differentially expressed miR. Verification was performed with advanced TaqMan assays in samples from all patients. Urine EV concentrations significantly increased in patients with AKI after surgery, with the percentage of EV positive for aquaporin-2 and β1-integrin also increasing. Pre surgery podocalyxin-positive EV were significantly lower, and β1-integrin EV werehigher in patients with AKI. The levels of the former correlated with the severity of theinjury. miR-125a-5p was expressed at higher levels in urine from patients with AKI stage 2/3. Levels of miR-10a-5p decreased after surgery in AKI patients; its levels correlated with the severity of the injury. Preoperative levels of podocalyxin EVs and miR-125a-5p had moderate AKI predictive value and, in a logistic model together with ICU lactate levels, offered good (AUC = 80.9%) AKI prediction. Conclusions: Lower levels of podocalyxin-positive EV at baseline predict the severity of post-surgery AKI. Urine EV concentrations and miR expression offer excellent predictive accuracy when combined with commonly measured biomarkers.

Dataset Information

Metabolomic interplay between the gut microbiome and plasma in CSA-AKI patients

Publications

Metabolomic interplay between gut microbiome and plasma metabolome in cardiac surgery-associated acute kidney injury.

Similar Datasets

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