Proteomics

Dataset Information

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Mass spectrometry identification of SUMOylated proteins pull down by Ni-NTA agarose.


ABSTRACT: In this project, we stably expressed His-SUMO3 in control and SENP1-depleted HEK293T cells. After the cells were infected with Sendai virus, we lysed cells by 8 M urea, purified SUMO3-associated proteins through His-tag pulldown using Ni-NTA agarose, and then utilized mass spectrometry to identify SUMOylated proteins in control and SENP1-depleted cells.

ORGANISM(S): Homo Sapiens

SUBMITTER: Fangfang Zhou  

PROVIDER: PXD037467 | iProX | Fri Oct 14 00:00:00 GMT+01:00 2022

REPOSITORIES: iProX

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