Proteomics

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Proteomic response of S. aureus to alpha-mangostin

ABSTRACT: S. aureus isolate was inoculated and cultured to exponential growth phase (OD600 at 0.5) in TSB and divided into two groups, the control group and the drug treated group. alpha-mangostin was added to the drug treated group until a final concentration of 1/2× MIC reached, and DMSO added in the control group. Then, cultures in both groups were cultivated for 2h at 180rpm. The microbial was centrifuged at 12000rpm for 10min, washed twice with Phosphate Buffer Saline (PBS). Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer.

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Zewen Wen  

PROVIDER: PXD037518 | iProX | Mon Oct 17 00:00:00 BST 2022

REPOSITORIES: iProX

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Mechanisms of Rapid Bactericidal and Anti-Biofilm Alpha-Mangostin <i>In Vitro</i> Activity against <i>Staphylococcus aureus</i>.

Deng Xiangbin X   Xu Hongbo H   Li Duoyun D   Chen Jinlian J   Yu Zhijian Z   Deng Qiwen Q   Li Peiyu P   Zheng Jinxin J   Zhang Haigang H  

Polish journal of microbiology 20230614 2

Alpha-mangostin (α-mangostin) was discovered as a potent natural product against Gram-positive bacteria, whereas the underlying molecular mechanisms are still unclear. This study indicated that α-mangostin (at 4 × MIC) rapidly killed <i>Staphylococcus aureus</i> planktonic cells more effectively (at least 2-log<sub>10</sub> CFU/ml) than daptomycin, vancomycin and linezolid at 1 and 3 h in the time-killing test. Interestingly, this study also found that a high concentration of α-mangostin (≥4×MIC  ...[more]

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