Dataset Information

The presence of HearNPV P26 in hemolymph from the virus-infected insect via liquid chromatography tandem mass spectrometry analysis

ABSTRACT: To examine whether P26 is involved in the extracellular melanization pathway, we used HearNPV and cotton bollworm as a testing system and detected the presence of HearNPV P26 in hemolymph from the virus-infected insect via liquid chromatography tandem mass spectrometry analysis. P26 was one of the most abundant viral proteins detected in the infected hemolymph at 48 and 72 h post infection, implying a role in the host hemolymph.

ORGANISM(S): Helicoverpa Armigera Nucleopolyhedrovirus G4

SUBMITTER: Zhihong Hu

PROVIDER: PXD037784 | iProX | Fri Oct 28 00:00:00 BST 2022

REPOSITORIES: iProX

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Publications

Dual roles and evolutionary implications of P26/poxin in antagonizing intracellular cGAS-STING and extracellular melanization immunity.

Yin Mengyi M Kuang Wenhua W Wang Qianran Q Wang Xi X Yuan Chuanfei C Lin Zhe Z Zhang Huanyu H Deng Fei F Jiang Haobo H Gong Peng P Zou Zhen Z Hu Zhihong Z Wang Manli M

Nature communications 20221114 1

P26, a homolog of the viral-encoded nuclease poxin that neutralizes the cGAS-STING innate immunity, is widely distributed in various invertebrate viruses, lepidopteran insects, and parasitoid wasps. P26/poxin from certain insect viruses also retains protease activity, though its biological role remains unknown. Given that many P26s contain a signal peptide, it is surmised that P26 may possess certain extracellular functions. Here, we report that a secretory baculoviral P26 suppresses melanizatio ...[more]

PMID: 36376305

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Project description:Citrus greening disease, also known as Huanglongbing (HLB), is the most serious disease of citrus plants. It is associated with the Gram-negative bacterium ‘Candidatus Liberibacter asiaticus’ (CLas), which is transmitted between host plants by the hemipteran insect vector Diaphorina citri in a circulative, propagative manner involving specific interactions with various insect tissues, including the hemolymph, fluid that occupies the body cavity akin to insect blood. High resolution quantitative mass spectrometry was performed to investigate the effect of CLas exposure on D. citri hemolymph at the proteome level. In contrast to the broad proteome effects on hundreds of proteins and a diverse array of metabolic pathways previously reported in gut and whole insect proteome analyses, the effect of CLas on the hemolymph was observed to be highly specific, restricted to key immunity and metabolism pathways, and lower in magnitude than that previously observed in the whole insect body and gut. Vitellogenins were abundantly expressed and CLas-responsive. Gene-specific RNA expression analysis suggests that these proteins are expressed in both male and female insects, and may have roles outside of reproductive vitellogenesis. Proteins for fatty acid synthesis were found to be up-regulated, along with metabolic proteins associated with energy production, supported at the organismal level by the previously published observation that D. citri individuals experience a higher level of hunger when reared on CLas-infected plants. Prediction of post-translational modifications identified hemolymph proteins with phosphorylation and acetylation upon CLas exposure. Proteins derived from the three most prominent bacterial endosymbionts of the psyllid were also detected in the hemolymph, and several of these have predicted secretion signals. A DNAK protein, the bacterial HSP70, detected in the hemolymph expressed from Wolbachia pipientis was predicted to encode a eukaryotic nuclear localization signal. Taken together, these data show specific changes to immunity and metabolism in D. citri hemolymph involving host and endosymbiont proteins. These data provide a novel context for proteomic changes seen in other D. citri tissues in response to CLas and align with organismal data on the effects of CLas on D. citri metabolism and reproduction.

Dataset Information

The presence of HearNPV P26 in hemolymph from the virus-infected insect via liquid chromatography tandem mass spectrometry analysis

Publications

Dual roles and evolutionary implications of P26/poxin in antagonizing intracellular cGAS-STING and extracellular melanization immunity.

Similar Datasets

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