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LC–MS hits of NcGrx5 proximal proteins by using BioID

ABSTRACT: NcGRX5-BirA* or Nc1 parasites were grown in medium containing 160 μM biotin for 24 h. The parasites were then harvested and lysed in RIPA buffer. The biotinylated proteins were captured using streptavidin magnetic beads. Mass spectrometry was used for the biotinylated proteins the biotinylated proteins.

ORGANISM(S): Neospora Caninum

SUBMITTER: Xingju Song

PROVIDER: PXD037871 | iProX | Tue Nov 01 00:00:00 GMT 2022

REPOSITORIES: iProX

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Regulation of Mitochondrial Energy Metabolism by Glutaredoxin 5 in the Apicomplexan Parasite Neospora caninum.

Song Xingju X Yang Xu X Ying Zhu Z Wu Kaijian K Liu Jing J Liu Qun Q

Microbiology spectrum 20221221 1

Iron-sulfur [Fe-S] clusters are one of the most ancient and functionally versatile natural biosynthetic prosthetic groups required by various proteins involved in important metabolic processes, including the oxidative phosphorylation of proteins, electron transfer, energy metabolism, DNA/RNA metabolism, and protein translation. Apicomplexan parasites harbor two possible [Fe-S] cluster assembly pathways: the iron-sulfur cluster (ISC) pathway in the mitochondria and the sulfur formation (SUF) path ...[more]

PMID: 36541793

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