Proteomics

Dataset Information

0

Identification of E. coli CheZ interactors by AP-MS


ABSTRACT: To identify E. coli CheZ interactors, affinity purification (AP) coupled with mass spectrometry (MS) was applied.

ORGANISM(S): Escherichia Coli

SUBMITTER: Shengce Tao  

PROVIDER: PXD037953 | iProX | Fri Nov 04 00:00:00 GMT 2022

REPOSITORIES: iProX

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Publications


Protein-biomolecule interactions play pivotal roles in almost all biological processes. For a biomolecule of interest, the identification of the interacting protein(s) is essential. For this need, although many assays are available, highly robust and reliable methods are always desired. By combining a substrate-based proximity labeling activity from the pupylation pathway of Mycobacterium tuberculosis and the streptavidin (SA)-biotin system, we developed the Specific Pupylation as IDEntity Repor  ...[more]

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