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Identification of ADAR1 associated proteins

ABSTRACT: To understand how ADAR1 controls genome stability, affinity purification and mass spectrometry were employed to identify the interactomes of ADAR1 with cellular extracts from HeLa cells stably expressing FLAG-ADAR1.Mass spectrometry analysis of FLAG-ADAR1 associated proteins. The differential protein bands of the immunoprecipitates from cellular extracts of HeLa cells were retrieved from silver stained SDS-PAGE, digested with trypsin and analyzed by mass spectrometry.

ORGANISM(S): Homo Sapiens

SUBMITTER: Lei Shi

PROVIDER: PXD038167 | iProX | Tue Nov 15 00:00:00 GMT 2022

REPOSITORIES: iProX

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ADAR1 links R-loop homeostasis to ATR activation in replication stress response.

Zhang Biao B Li Yi Y Zhang Jieyou J Wang Yuejiao Y Liang Can C Lu Ting T Zhang Chunyong C Liu Ling L Qin Yan Y He Jiahuan J Zhao Xiangnan X Yu Jia J Hao Jihui J Yang Jie J Li Mulin Jun MJ Yao Zhi Z Ma Shuai S Cheng Hui H Cheng Tao T Shi Lei L

Nucleic acids research 20231101 21

Unscheduled R-loops are a major source of replication stress and DNA damage. R-loop-induced replication defects are sensed and suppressed by ATR kinase, whereas it is not known whether R-loop itself is actively involved in ATR activation and, if so, how this is achieved. Here, we report that the nuclear form of RNA-editing enzyme ADAR1 promotes ATR activation and resolves genome-wide R-loops, a process that requires its double-stranded RNA-binding domains. Mechanistically, ADAR1 interacts with T ...[more]

PMID: 37831098

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