Dataset Information

Proteomic analysis of Macleaya cordata leaf under excess zinc

ABSTRACT: The objectives of this study were to investigate response and tolerance of Macleaya cordata to excess zinc based on comparative analysis of proteome.

ORGANISM(S): Macleaya Cordata

SUBMITTER: Hongxiao Zhang

PROVIDER: PXD038219 | iProX | Mon Nov 21 00:00:00 GMT 2022

REPOSITORIES: iProX

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Publications

Response and Tolerance of Macleaya cordata to Excess Zinc Based on Transcriptome and Proteome Patterns.

Zhang Hongxiao H Hu Linfeng L Du Xinlong X Shah Assar Ali AA Ahmad Baseer B Yang Liming L Mu Zhiying Z

Plants (Basel, Switzerland) 20230611 12

Macleaya cordata is a dominant plant of mine tailings and a zinc (Zn) accumulator with high Zn tolerance. In this study, M. cordata seedlings cultured in Hoagland solution were treated with 200 μmol·L-1 of Zn for 1 day or 7 days, and then, their leaves were taken for a comparative analysis of the transcriptomes and proteomes between the leaves of the control and Zn treatments. Differentially expressed genes included those that were iron (Fe)-deficiency-induced, such as v ...[more]

PMID: 37375899

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Project description:This a model from the article: The dynamic balance of import and export of zinc in Escherichia coli suggests a heterogeneous population response to stress Takahashi H, Oshima T, Doherty N, Clayton SR, Iqbal M, Hill PJ, Hobman JL, Tobe T, Ogasawara N, Kanaya S and Stekel DJ journal year id, Abstract: Zinc is essential for life, but toxic in excess. Thus all cells must control their internal zinc concentration. We used a systems approach, alternating rounds of experiments and models, to further elucidate the zinc control systems in Escherichia coli. We measured the response to zinc of the main specific zinc import and export systems in the wild type, and a series of deletion mutant strains. We interpreted these data with a detailed mathematical model and Bayesian model fitting routines. There are three key findings: First, that alternate, non-inducible importers and exporters are important. Second, that an internal zinc reservoir is essential for maintaining the internal zinc concentration. Third, our data fitting led us to propose that the cells mount a heterogeneous response to zinc: some respond effectively, while others die or stop growing. In a further round of experiments we demonstrated lower viable cell counts in the mutant strain tested exposed to excess zinc, consistent with this hypothesis. A stochastic model simulation demonstrated considerable fluctuations in the cellular levels of the ZntA exporter protein, reinforcing this proposal. We hypothesise that maintaining population heterogeneity could be a bet-hedging response allowing a population of cells to survive in varied and fluctuating environments. This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2011 The BioModels.net Team. For more information see the terms of use. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le NovÃ¨re N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.

Dataset Information

Proteomic analysis of Macleaya cordata leaf under excess zinc

Publications

Response and Tolerance of <i>Macleaya cordata</i> to Excess Zinc Based on Transcriptome and Proteome Patterns.

Similar Datasets

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