Staphylococcus aureus biofilm quantitative proteomics comparative study to observe the global expression of protein DMSO treatment group and radezolid treatment group
ABSTRACT: S. aureus isolate was inoculated and cultured to exponential growth phase (OD600 at 0.5) in TSB and divided into two groups, the control group and the drug treated group. Radezolid was added to the drug treated group until a final concentration of 1/8× MIC reached, and DMSO added in the control group. Then, cultures in both groups were cultivated for 2 h at 200 rpm. The microbial was centrifuged at 12000 rpm for 10 min, washed twice with Phosphate Buffer Saline (PBS). Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer.
ORGANISM(S): Staphylococcus Aureus
SUBMITTER: Zhijian Yu
PROVIDER: PXD038354 | iProX | Fri Nov 25 00:00:00 GMT 2022