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A DIA-based deep proteome profiling of Pseudomonas aeruginosa PAO1 reference strain under different growth conditions

ABSTRACT: We used the data-independent acquisition (DIA)-based quantitative proteomics to profile the abundance dynamics of proteins across different growth stages in P. aeruginosa PAO1 reference strain. Extensive modulations of protein expression levels were found in planktonic culture of PAO1 bacteria across different growth stages as well as when comparing the protein expressions in biofilm cells to planktonic cells.

ORGANISM(S): Pseudomonas Aeruginosa Pao1

SUBMITTER: Lingyun Dai

PROVIDER: PXD038460 | iProX | Thu Apr 06 00:00:00 BST 2023

REPOSITORIES: iProX

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Deep Profiling of the Proteome Dynamics of Pseudomonas aeruginosa Reference Strain PAO1 under Different Growth Conditions.

Hou Mengyun M Huang Jingnan J Jia Tianyuan T Guan Yudong Y Yang Fan F Zhou Hongchao H Huang Piying P Wang Jigang J Yang Liang L Dai Lingyun L

Journal of proteome research 20230522 6

As one of the most common bacterial pathogens causing nosocomial infections, Pseudomonas aeruginosa is highly adaptable to survive under various conditions. Here, we profiled the abundance dynamics of 3489 proteins across different growth stages in the P. aeruginosa reference strain PAO1 using data-independent acquisition-based quantitative proteomics. The proteins differentially expressed during the planktonic growth exhibit several distinct patterns of expression profiles and are ...[more]

PMID: 37212837

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