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Systematic profiling of lysine lactylation proteome reveals distinct changes in lactylation between the parental AGS cells and cisplatin-resistant AGS cells

ABSTRACT: Lactate enable to cause a novel post-translational modification, lactylation of proteins. We are interested in exploring whether lactate modulates DNA-damaging agents resistance in the form of lactylation. To gain a global view of DNA-damaging agents resistance-related lactylation, especially Kla of nonhistone substrates, we used 4D-Label free high-resolution LC-MS/MS, quantitative lysine lactylation analysis to investigate Kla substrates in cisplatin-resistant AGS cells.

ORGANISM(S): Homo Sapiens

SUBMITTER: Hengxing Chen

PROVIDER: PXD038880 | iProX | Fri Dec 16 00:00:00 GMT 2022

REPOSITORIES: iProX

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Systematic profiling of lysine lactylation proteome reveals distinct changes in lactylation between the parental AGS cells and cisplatin-resistant AGS cells

Project description:Lactate enable to cause a novel post-translational modification, lactylation of proteins. We are interested in exploring whether lactate modulates DNA-damaging agents resistance in the form of lactylation. To gain a global view of DNA-damaging agents resistance-related lactylation, especially Kla of nonhistone substrates, we used 4D-Label free high-resolution LC-MS/MS, quantitative lysine lactylation analysis to investigate Kla substrates in cisplatin-resistant AGS cells.

2024-05-09 | PXD050906 | Pride

Metabolic regulation of gene expression by histone lactylation

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HBO1 catalyzes lysine lactylation and mediates histone H3K9la to regulate gene transcription

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Hexokinase 2-mediated gene expression via histone lactylation is required for hepatic stellate cell activation and liver fibrosis (CUT&Tag)

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2023-05-05 | GSE229154 | GEO

Hexokinase 2-mediated gene expression via histone lactylation is required for hepatic stellate cell activation and liver fibrosis (HSC-RNA-Seq)

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Project description:Lysine lactylation (Kla) is a newly discovered posttranslational modification showing the addition of an L-lactate-derived lactyl group to the lysine residue. Histone lactylation has been found to regulate the downstream genes expression involved in the process of infection clearance, homeostasis retore, and tumorigenesis. Like other metabolite mediated PTMs, which the cellular levels of these metabolites affect the stoichiometry of the corresponding PTMs, the level of Kla also responds to the intracellular lactate concentration in a dose-dependent fashion associated with glycolysis metabolism. It has long been noted that manipulation of glycolysis metabolism could affect the tendon cell function, tendon homeostasis, and healing process of tendon.Nonetheless, the lactylation sites and regulations in tendinopathy remain unexplored.

2023-05-10 | PXD033146 | Pride

Ikzf1 lactylation regulates CD4 T cell differentiation

Project description:Dysregulation of Th17 differentiation was implicated in multiple inflammatory and autoimmune diseases including autoimmune uveitis. In the current study, we have provided evidence indicating that lactate-derived lactylation plays important roles in regulating Th17 differentiation. The lactylation level of CD4+ T cells was upregulated in EAU mice and inhibiting lactylation resulted in impaired EAU progression. We characterized the global lactylome of CD4+ T cells of normal and EAU mice. We found that the differentially lactylated proteins were enriched in pathways related to immune responses including leukocyte differentiation. Importantly, our results show that the lactylation level of Ikzf1 (K164) functions in regulating Th17 differentiation by differentially modulating gene expression patterns which are related to CD4+ T cell differentiation by CUT& Tag analysis. In view of the above mentioned well-documented evidence, Ikzf1 lactylation might represent an important regulator for Th17 differentiation in autoimmune uveitis.

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