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Identification of SNHG15 Binding Protein in SNHG15 overexpression BT549 cells Using MS2 Pull down and Mass Spectrometry

ABSTRACT: SNHG15 is an oncogenic lncRNA in a variety of cancers including breast cancer, lung cancer, and hepatocellular carcinoma. In the present study, We used MS2 pulldown and MS spectrometry to Identification of SNHG15 Binding Protein in SNHG15 overexpression BT549 cells.

ORGANISM(S): Homo Sapiens

SUBMITTER: Wei Gu

PROVIDER: PXD039100 | iProX | Tue Dec 27 00:00:00 GMT 2022

REPOSITORIES: iProX

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SNHG15-Mediated Localization of Nucleolin at the Cell Protrusions Regulates CDH2 mRNA Expression and Cell Invasion.

Chen Shaoying S Zhou Yanchun Y Peng Pei P Xu Liqun L Tang Quandong Q Chen Weibin W Gu Wei W

International journal of molecular sciences 20231026 21

LncRNAs are emerging as important regulators of gene expression by controlling transcription in the nucleus and by modulating mRNA translation in the cytoplasm. In this study, we reveal a novel function of lncRNA SNHG15 in mediating breast cancer cell invasion through regulating the local translation of CDH2 mRNA. We show that SNHG15 preferentially localizes at the cellular protrusions or cell leading edge and that this localization is directed by IMP1, a multifunctional protein involved in many ...[more]

PMID: 37958584

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