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Proteins interacting with SARS-CoV-2 envelope

ABSTRACT: HEK293T were transfected with SARS-CoV-2-E-HA for 36 h, then treated with MG132 or DMSO for 12 h prior to harvest. Co-IP assay were performed with HA beads (Roche, catalog no. 11867423001), the elution was analyzed by mass spectrum. Mass spectrum analysis were performed by the national center for protein science (Beijing, CHN).

ORGANISM(S): Homo Sapiens

SUBMITTER: Wenyan Zhang

PROVIDER: PXD039140 | iProX | Sun Jan 01 00:00:00 GMT 2023

REPOSITORIES: iProX

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The E3 ligase RNF5 restricts SARS-CoV-2 replication by targeting its envelope protein for degradation.

Li Zhaolong Z Hao Pengfei P Zhao Zhilei Z Gao Wenying W Huan Chen C Li Letian L Chen Xiang X Wang Hong H Jin Ningyi N Luo Zhao-Qing ZQ Li Chang C Zhang Wenyan W

Signal transduction and targeted therapy 20230203 1

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a severe global health crisis; its structural protein envelope (E) is critical for viral entry, budding, production, and induction of pathology which makes it a potential target for therapeutics against COVID-19. Here, we find that the E3 ligase RNF5 interacts with and catalyzes ubiquitination of E on the 63rd lysine, leading to its degradation by the ubiquitin-prote ...[more]

PMID: 36737599

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