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Ciona savignyi embryos 4D Label-free Quantitative Phosphoproteomics

ABSTRACT: Formation of tubular system is crucial for embryogenesis and adult metabolism. However，the underlying mechanisms remain largely unclear. We demonstrated that a dual specificity tyrosine-phosphorylation-regulated kinase 1 (DYRK1), the protein kinase, which was upregulated during lumenogenesis and required for ascidian notochord extracellular lumen expansion. Here, we applied 4D Label-free Quantitative Phosphoproteomics and aimed to find out the potential substrates (phosphoproteins) that were regulated by DYRK1during ascidian Ciona notochord lumen expansion.

ORGANISM(S): Ciona Savignyi

SUBMITTER: Bo Dong

PROVIDER: PXD039234 | iProX | Wed Jan 04 00:00:00 GMT 2023

REPOSITORIES: iProX

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DYRK1-mediated phosphorylation of endocytic components is required for extracellular lumen expansion in ascidian notochord.

Ouyang Xiuke X Wu Bingtong B Yu Haiyan H Dong Bo B

Biological research 20230311 1

<h4>Background</h4>The biological tube is a basal biology structure distributed in all multicellular animals, from worms to humans, and has diverse biological functions. Formation of tubular system is crucial for embryogenesis and adult metabolism. Ascidian Ciona notochord lumen is an excellent in vivo model for tubulogenesis. Exocytosis has been known to be essential for tubular lumen formation and expansion. The roles of endocytosis in tubular lumen expansion remain largely unclear.<h4>Results ...[more]

PMID: 36899423

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