In vitro kinase assay to detect STX17 phosphorylation by ULK1

ABSTRACT: To identify the specific site of STX17 phosphorylated by ULK, in vitro kinase assays were performed with ULK1 immunoprecipitated from 293T cells and STX17 purified from E. coli. Purified GST-STX17 proteins were used for kinase assay as substrates to determine phosphorylation sites.

ORGANISM(S): Homo Sapiens

SUBMITTER: Yueguang Rong  

PROVIDER: PXD039362 | iProX | Tue Jun 27 00:00:00 BST 2023

REPOSITORIES: iProX