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Proteomics of exosomes of HUVECs supernatant

ABSTRACT: The data were analyzed by Meiji Biotechnology (Shanghai, China). Briefly, Tandem mass tags (TMT) quantification-based proteomics was used to analyze the expression level of different proteins to reveal the mechanism of CD151 on cardiac function. Proteins were extracted by 8 M ureophilplus 1% SDS, which were then alkylated, hydrolyzed by trypsin, and mixed with TMT buffer (ThermoFisher, USA). The labeled peptide fragment was analyzed by an ultra-performance liquid chromatography (UPLC)-MS/MS system. The fragmentations were identified by comparing the spectrum with the database using Proteome Discoverer TM Software 2.4

ORGANISM(S): Homo Sapiens

SUBMITTER: Houjuan Zuo

PROVIDER: PXD039733 | iProX | Sun Jan 29 00:00:00 GMT 2023

REPOSITORIES: iProX

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