Dataset Information

4D label free proteomics of membrane vesicles of RN4220 and the mutants RN4220-ΔHld and RN4220-ΔagrA

ABSTRACT: The 4D label free proteomics of membrane vedsicles (MVs) of Staphylococcus aureus RN4220 and the mutants RN4220-ΔHld, which the 20th base “C” in the hld gene was changed to “G” to achieve a stop codon TGA and result in a truncated translation of Hld, and RN4220-ΔagrA, which agrA gene was deleted.

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Xiancai Rao

PROVIDER: PXD039734 | iProX | Sun Jan 29 00:00:00 GMT 2023

REPOSITORIES: iProX

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Publications

Loaded delta-hemolysin shapes the properties of <i>Staphylococcus aureus</i> membrane vesicles.

Chen Juan J Lv Yuhuan Y Shang Weilong W Yang Yi Y Wang Yuting Y Hu Zhen Z Huang Xiaonan X Zhang Rong R Yuan Jizhen J Huang Jingbin J Rao Xiancai X

Frontiers in microbiology 20231006

<h4>Background</h4>Membrane vesicles (MVs) are nanoscale vesicular structures produced by bacteria during their growth <i>in vitro</i> and <i>in vivo</i>. Some bacterial components can be loaded in bacterial MVs, but the roles of the loaded MV molecules are unclear.<h4>Methods</h4>MVs of <i>Staphylococcus aureus</i> RN4220 and its derivatives were prepared. Dynamic light scattering analysis was used to evaluate the size distribution, and 4D-label-free liquid chromatography-tandem mass spectromet ...[more]

PMID: 37869662

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Project description:miRNA levels depend on both biogenesis and turnover. The methyltransferase HEN1 stabilizes plant miRNAs, animal piRNAs, and siRNAs in both kingdoms via 3' terminal methylation. Loss of HEN1 in plants results in non-templated oligo-uridylation and accelerated degradation of miRNAs. In hen1 mutants from Arabidopsis and rice, we found that the patterns of miRNA truncation and uridylation differ substantially among miRNA families, but such patterns for the same miRNA are conserved between species. miR166 and miR163 are truncated predominantly to ~17 and ~16 nt, and subsequently recover via uridylation to approximately their original sizes, 21 and 24 nt, suggesting that in these cases miRNA truncation triggers uridylation. miR171 is untruncated but uridylated to 22 nt in hen1 mutants, gaining the ability to trigger production of phased, secondary siRNAs. Truncated and tailed variants were bound by ARGONAUTE1 (AGO1) in hen1, implying that these events occur while miRNAs are still bound by AGO1. Unexpectedly, a portion of miR158 in wildtype remains unmethylated and thus subject to uridylation and destabilization, suggesting that plants naturally utilize miRNA methylation to modulate miRNA accumulation. Our results suggest that the AGO1-containing RISC complex may undergo programming to reflect each bound miRNA, determining a defined, distinct decay destiny. In this analysis, we sequenced sRNAs from two hen1 mutant alleles in Arabidopsis and three hen1 alleles in rice. In Arabidopsis, the strong hen1-1 allele in the Landsberg erecta (Ler) ecotype is the first hen1 mutant and emerged from an enhancer screen in the hua1-1/hua2-1 background, and hen1-8 in the Columbia (Col) background is a weak allele. In rice, WAVY LEAF1 (WAF1) is the ortholog of Arabidopsis HEN1, and two mutant alleles waf1-1 and waf1-2 each bear a single-base substitution leading to a premature stop codon in the second exon and a non-functional splicing site of the fourth intron, respectively. We identified a third mutant allele of the rice HEN1 gene (Oshen1-3 from the Korean (POSTEC) rice T-DNA mutant population).

Dataset Information

4D label free proteomics of membrane vesicles of RN4220 and the mutants RN4220-ΔHld and RN4220-ΔagrA

Publications

Loaded delta-hemolysin shapes the properties of <i>Staphylococcus aureus</i> membrane vesicles.

Similar Datasets

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