Dataset Information

In-depth proteomic analyzing the activated effect and sensitized mechanism of clinical multi-drug resistant of Pseudomonas aeruginosa by TanReQing injection

ABSTRACT: This study demonstrates for the first time that a number of important peptides associated with MDR-PA resistance and the resistance-related unique proteins associated with TRQ treatment support resistance sensitization potentiation in the MDR-PA.

ORGANISM(S): Pseudomonas Aeruginosa Aes-1r

SUBMITTER: Xianyu Li

PROVIDER: PXD039911 | iProX | Tue Feb 07 00:00:00 GMT 2023

REPOSITORIES: iProX

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In-depth analysis of the treatment effect and synergistic mechanism of TanReQing injection on clinical multi-drug resistant Pseudomonas aeruginosa.

Li Dongying D Li Yueyi Y Wang Jingyi J Yang Weifeng W Cui Kaiyu K Su Renjing R Li Lu L Ren Xing X Li Xianyu X Wang Yi Y

Microbiology spectrum 20240228 4

Antibiotic resistance is a recognized and concerning public health issue. Gram-negative bacilli, such as Pseudomonas aeruginosa (P. aeruginosa), are notorious for their rapid development of drug resistance, leading to treatment failures. TanReQing injection (TRQ) was chosen to explore its pharmacological mechanisms against clinical multidrug-resistant P. aeruginosa (MDR-PA), given its antibacterial and anti-inflammatory properties. We revealed the expression of proteins and ...[more]

PMID: 38415603

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Project description:BackgroundThe biofilm state of pathogens facilitates antimicrobial resistance which makes difficult-to-treat infections. In this regard, it has been found that the compounds screened from plant extracts represent one category of the most promising antibiofilm agents. However, the antibiofilm activities and the active ingredients of plant extracts remain largely unexplored. In this background, the study is (1) to screen out the plant extracts with antibiofilm ability against Pseudomonas aeruginosa, and (2) to identify the active ingredients in the plant extracts and elucidate the underlying mechanism of the antibiofilm activities.MethodsMicro-broth dilution method, in vitro biofilm model, LC-MS/MS analysis and P. aeruginosa-mouse infection model were adopted to assess the antibiofilm activity. GC-MS analysis was performed to detect the active ingredients in plasma. RNA-Seq, GO analysis, KEGG analysis and RT-qPCR were adopted to elucidate the underlying mechanism of antibiofilm activities against P. aeruginosa.ResultsLonicerae Japonicae Flos (LJF) among 13 plants could exert significant inhibitory effects on bacterial biofilm formation, mobility and toxin release in vitro, and it could exert antibiofilm effect in vivo too. Moreover, quinic acid, as one metabolite of chlorogenic acid, was found as an active ingredient in LJF against the biofilm of P. aeruginosa. The active ingredient significantly inhibited EPS secretion in biofilm formation and maturity and could achieve synergistic antibiofilm effect with levofloxacin. It reduced the biofilm formation by regulating core targets in quorum sensing system. In GO process, it was found that the core targets were significantly enriched in multiple biological processes involving locomotion, chemotaxis and motility mediated by flagellum/cilium, which was related to KEGG pathways such as bacterial chemotaxis, oxidative phosphorylation, ribosome, biofilm formation, cyanoamino acid metabolism and quorum sensing. Finally, the binding of quinic acid with core targets rhlA, rhlR and rhlB were validated by molecular docking and RT-qPCR.ConclusionsIn summary, the study verified the in vitro and in vivo antibiofilm effects of LJF against P. aeruginosa and elucidated the active ingredients in LJF and its conceivable pharmacological mechanism, indicating that quinic acid could have the potential of an antibiofilm agent against P. aeruginosa and related infections.

Dataset Information

In-depth proteomic analyzing the activated effect and sensitized mechanism of clinical multi-drug resistant of Pseudomonas aeruginosa by TanReQing injection

Publications

In-depth analysis of the treatment effect and synergistic mechanism of TanReQing injection on clinical multi-drug resistant <i>Pseudomonas aeruginosa</i>.

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