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Quantitative Proteomics Assay of G Protein-Coupled Receptor Kinase4 in hepatocellualr carcinoma Cell Growth

ABSTRACT: A quantitative proteomics assay was used to analyze the protein profile and screen differentially expressed proteins (DEPs) between the GRK4 overexpression with lentivirus vector(OE) and normal control lentivirus vector (NC) HepG2 cells.

ORGANISM(S): Homo Sapiens

SUBMITTER: Yunxiu Luo

PROVIDER: PXD039951 | iProX | Wed Feb 08 00:00:00 GMT 2023

REPOSITORIES: iProX

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Quantitative proteomics assay reveals G protein-coupled receptor kinase 4-induced HepG2 cell growth inhibition.

Luo Yunxiu Y Yang Jing J Wang Yan Y

Heliyon 20240412 8

<h4>Background and aim</h4>To investigate the biological effects and putative biological mechanism of G protein-coupled receptor kinase 4 (GRK4) on HepG2 cells.<h4>Materials and methods</h4>Cell proliferation, cycle, and apoptosis were evaluated by Cell Counting Kit-8 and flow cytometry (FCM) in HepG2 cells infected with either the GRK4-overexpressing lentivirus vector (OE) or the negative control lentivirus vector (NC). The protein profiles and differentially expressed proteins (DEPs) of the OE ...[more]

PMID: 38638965

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