Proteomics

Dataset Information

0

Cerebellum PC O-GlcNAcylation TMT analysis

ABSTRACT: The total protein in the sample was extracted, part of the sample was taken out for protein concentration determination and SDS-PAGE detection, the other part was taken out for trypsin enzymolysis and labeling, and then equal amount of each labeled sample was mixed for chromatographic separation. Finally, the sample was analyzed by LC-MS/MS and data analysis. After quality assessment and preprocessing, expression level analysis and function analysis were conducted respectively. Functional annotation analysis of the identified proteins was performed using several common databases. GO analysis, Pathway analysis and interaction analysis were conducted for the screened differential proteins, and correlation analysis, expression pattern clustering heat map and venn analysis were conducted for the data of the comparison group. In addition, according to the data situation, study the correlation or the part of interest, select the key proteins and their functions or pathways, and conduct subsequent key research and verification direction.

ORGANISM(S): Mus Musculus

SUBMITTER: Haitao Wu  

PROVIDER: PXD040011 | iProX | Thu Feb 09 00:00:00 GMT 2023

REPOSITORIES: iProX

Json Xml
altmetric image

Publications

O-GlcNAcylation Is Required for the Survival of Cerebellar Purkinje Cells by Inhibiting ROS Generation.

Liu Fengjiao F   Li Shen S   Zhao Xin X   Xue Saisai S   Li Hao H   Yang Guochao G   Li Ying Y   Wu Yan Y   Zhu Lingling L   Chen Liping L   Wu Haitao H  

Antioxidants (Basel, Switzerland) 20230326 4

Purkinje cells (PCs), as a unique type of neurons output from the cerebellar cortex, are essential for the development and physiological function of the cerebellum. However, the intricate mechanisms underlying the maintenance of Purkinje cells are unclear. The O-GlcNAcylation (O-GlcNAc) of proteins is an emerging regulator of brain function that maintains normal development and neuronal circuity. In this study, we demonstrate that the O-GlcNAc transferase (OGT) in PCs maintains the survival of P  ...[more]

Similar Datasets

Proteomics Gated phosphorylated proteomics of LAIR1-overexpressed and LAIR1-control in GL261 cells
2023-06-13 | PXD042932 |
Transcriptomics To determine whether AhR KO has an effect on cell differentiation and/or global gene expression profiles in individual sorted colonic stem cells
2021-02-27 | GSE167595 | GEO
Transcriptomics Gene expression profiling of negative-pressure-treated split-thickness skin graft donor site wounds reveals novel effects on epithelialization
2011-10-25 | GSE33169 | GEO
Transcriptomics Global expression analysis of differential expression of genes working under Soil Water Stress condition in different genotypes of rice in Vegetative and Grain-filling stage [set 1]
2016-04-01 | GSE49364 | GEO
Genomics Using lncRNA sequencing to reveal a putative lncRNA-mRNA correlation network and potential role of PCBP1-AS1 in pathogenesis of cervical cancer
2021-02-24 | GSE167362 | GEO
Transcriptomics Control of gene expression mRNA N6-methyladenosine in pro-B cells
2020-06-22 | GSE151071 | GEO
Transcriptomics Expression data from primary tissue, human oral mucosa (mRNA data)
2013-05-06 | GSE38616 | GEO
Genomics Expression data from primary tissue, human oral mucosa (miRNA data)
2013-05-06 | GSE38615 | GEO
Transcriptomics Genetic analysis of residual feed intake and its molecular pathways in coloured broiler chicken [duodenum]
2018-07-12 | GSE116940 | GEO
Transcriptomics Genetic analysis of residual feed intake and its molecular pathways in coloured broiler chicken [liver]
2018-07-12 | GSE116932 | GEO