Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

SRNA SNC251 participates in hyperosmotic stress response regulation in Sphingomonas melonis TY

ABSTRACT: The wild type (WT) of S. melonis TY and snc251 deleted cells carrying either a plasmid overexpressing SNC251 (Δsnc251 comp.), or an empty control vector (Δsnc251) were grown in Luria-Bertani (LB) culture to an OD600 of 0.6.

ORGANISM(S): Sphingomonas Melonis Ty

SUBMITTER: Zhenmei Lu

PROVIDER: PXD040071 | iProX | Sat Feb 11 00:00:00 GMT 2023

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

sRNA SNC137 regulation in Sphingomonas melonis TY

Project description:Proteome of the wild type (WT) of S. melonis TY and snc137 deleted cells carrying either a plasmid overexpressing SNC137 (Δsnc137 comp.), or an empty control vector (Δsnc137)

2023-02-13 | PXD040079 | iProX

sRNA137 regulation in Sphingomonas melonis TY

Project description:Transcriptome of the wild type (WT) of S. melonis TY and snc137 deleted cells carrying either a plasmid overexpressing SNC137 (Δsnc137 comp.), or an empty control vector (Δsnc137)

2023-02-24 | GSE224594 | GEO

Transcription profiling of E. coli K12 Bw25113/pCA24N-hha with 2 mM IPTG vs BW25113/pCA24N with 2 mM IPTG

Project description:gene expression profiles by overexpressing Hha from pCA24N-hha using 2 mM IPTG induction in BW25113 wild type suspension cells in LB medium at 37C relative to BW25113 carrying the empty vector plasmid in the same test conditions. Experiment Overall Design: Strains: E. coli K12 BW25113/pCA24N, BW25113/pCA24N-hha Experiment Overall Design: Growth conditions: grow culture in LB medium at 37C to OD600 0.1 and add 2 mM IPTG to induce, and grow to OD600 0.5 to collect cells. RNA was extraction, and gene expression profiles were evaluated.

2008-10-20 | E-GEOD-10116 | biostudies-arrayexpress

Overexpression of Sinorhizobium meliloti ECF sigma factors.

Project description:We characterized transcriptomes for strains overexpressing each of the Sinorhizobium meliloti ECF sigma factors the via a plasmid-borne, melibiose-inducible promoter plasmid (PmelA; pCAP11: Pinedo et al. 2008 J Bacteriol 190:2947-2956) compared to control strains carrying the empty vector.

2018-09-05 | GSE116665 | GEO

E. coli K12 Bw25113/pCA24N-hha with 2 mM IPTG vs BW25113/pCA24N with 2 mM IPTG

2008-06-17 | GSE10116 | GEO

Transcription profiling of Saccharomyces cerevisiae pGAL1-MKS1, pGAL1-NNK1, and pGAL1-FMP48 overexpression

Project description:Analysis of the transcriptional effects of pGAL1-MKS1, pGAL1-NNK1, and pGAL1-FMP48 overexpression. BY4700 gal1::NAT ura3delta0 S. cerevisiae cells carrying a [pGAL1-MKS1-HA, URA3, CEN], [pGAL1-NNK1-HA, URA3, CEN], or [pGAL1-FMP48-HA, URA3, CEN] plasmid, or empty vector BG1805, were grown to early log phase, induced with 0.2% galactose, and harvested after 1.5 h and 6 h. RNA from a strain overexpressing MKS1, NNK1, or FMP48 was competitively hybridized with RNA from an identically treated strain carrying empty vector.<br><br>FMP48 = YGR052W. MKS1 = YNL076W. NNK1 = YKL171W.

2010-05-25 | E-MEXP-2171 | biostudies-arrayexpress

Splicing and gene expression changes in human mammary epithelial MCF-10A cells expressing SR or SR-like proteins

Project description:RNA-seq data from human mammary epithelial MCF-10A cells expressing the coding sequence of SRSF2, SRSF4, SRSF6, SRSF9, TRA2B, or a control empty vector plasmid grown for 8 days in 3D culture in matrigel

2019-11-26 | GSE137440 | GEO

MGAS5005 rivRX knockout vs. rivRX overexpressing strain

Project description:JRS1616/pREG696 is our MGAS5005 covR rivRX double knockout carrying empty vector. JRS1616/pJRS1624 is our covR rivRX double knockout carrying a plasmid which overexpresses rivRX. Keywords: comparitive transcriptome analysis

2007-09-11 | GSE8940 | GEO

SLF1 overexpression versus control cells

Project description:Measure the relative changes of gene expression upon GIS2 overexpression; BY4741 cells bearing plasmid pBG1805-Slf1 or control plasmid pBG1805 were grown in SC ura- gal at 30 C for 6 or 24 hours. An overnight culture of cells was inoculated at an OD600 of 0.1 and further grown to an OD600 of 0.5- 0.7 in 60 ml SC Ura- Gal at 30 C. Cells were harvested by centrifugation, washed twice with 600 ul of ice-cold sterile water. 200 ul of cells were removed and RNA was isolated by hot phenol extraction for microarray analysis. 7.5 ug of total RNA derived from cells expressing the empty vector (pBG1805) or Slf1p (pBG1805_Slf1) were reverse transcribed in the presence of 5-(3-aminoallyl)-dUTP and natural dNTPs with a mixture of N9 and dT20V primers, and cDNAs were covalently linked to Cy3 and Cy5 NHS-monoesters (GE HealthSciences Cat# RPN5661), respectively, and competitively hybridized on yeast oligo arrays at 42 degrees celsius for 14 hours in formamide-based hybridization buffer. Microarrays were scanned with an Axonscanner 4200 (Molecular Devices) and analyzed with GenePix Pro 5.1 (Molecular devices).

2012-02-08 | GSE27311 | GEO

Evidence that TSC2 acts as a transcription factor and binds to and represses the promoter of Epiregulin

Project description:In order to identify the genes regulated by TSC2, gene expression profiling of two clones stably overexpressing TSC2 was performed. The gene expression profiles of two stable transfectant clones overexpressing the full-length TSC2 in KB cells were compared with the gene expression profile of a vector control clone carrying an empty plasmid.

2013-11-05 | E-GEOD-52061 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data