Identification of maize xylem sap
Ontology highlight
ABSTRACT:
ORGANISM(S): Zea Mays
SUBMITTER: 
Xinhua Zhan
PROVIDER: PXD040256 | iProX | Sat Feb 18 00:00:00 GMT 2023
REPOSITORIES: iProX
Similar Datasets
Project description:The differentially expressed proteins in wheat xylem sap after phenanthrene exposure were screened by the threshold (fold change≥1.5 for up-regulation or ≤ 0.67 for down-regulation, P<0.05). Peroxidase (A0A3B5XXD0) was significantly decreased after phenanthrene exposure (fold change≤0.67, P<0.05). But there were numerous and stable existents of peroxidase (A0A3B5XXD0) whether under phenanthrene exposure or not. The quantitative approach of the proteomic data analysis relied on the label-free quantification (LFQ) intensities calculated by MaxQuant.The large amount of peroxidase and the extraordinarily increased expression of uncharacterized protein under phenanthrene exposure led to promote the acropetal translocation of phenanthrene in wheat xylem sap.
2021-09-15 | PXD028208 | iProX
Project description:The differentially expressed proteins in soybean xylem sap after phenanthrene exposure were screened by the threshold (fold change≥1.5 for up-regulation or ≤ 0.67 for down-regulation, P<0.05). Peroxidase (A0A3B5XXD0) was significantly decreased after phenanthrene exposure (fold change≤0.67, P<0.05). But there were numerous and stable existents of peroxidase (A0A3B5XXD0) whether under phenanthrene exposure or not. The quantitative approach of the proteomic data analysis relied on the label-free quantification (LFQ) intensities calculated by MaxQuant.The large amount of peroxidase and the extraordinarily increased expression of uncharacterized protein under phenanthrene exposure led to promote the acropetal translocation of phenanthrene in wheat xylem sap.
2023-02-18 | PXD040258 | iProX
Project description:The differentially expressed proteins in wheat xylem sap after phenanthrene exposure were screened by the threshold (fold change≥1.5 for up-regulation or ≤ 0.67 for down-regulation, P<0.05). Peroxidase (A0A3B5XXD0) was significantly decreased after phenanthrene exposure (fold change≤0.67, P<0.05). But there were numerous and stable existents of peroxidase (A0A3B5XXD0) whether under phenanthrene exposure or not. The quantitative approach of the proteomic data analysis relied on the label-free quantification (LFQ) intensities calculated by MaxQuant.The large amount of peroxidase and the extraordinarily increased expression of uncharacterized protein under phenanthrene exposure led to promote the acropetal translocation of phenanthrene in wheat xylem sap.
2021-09-01 | PXD028203 | iProX
Project description:The aim of this work was to study the effects of Mn excess on the protein profile of the xylem sap of tomato (Solanum lycopersicum), with the aim of elucidating plant response mechanisms to these nutritional stresses. Tomato was chosen as a model plant because the tomato genome has been published and this plant species has adequate root pressure and turgid stems that permit xylem sap sampling in sufficient amounts by de-topping. The high- throughput shotgun analysis has permitted to identify and quantitate a large number of low abundance proteins in the tomato xylem sap.
2020-12-10 | PXD021973 | Pride
Project description:The aim of this work was to study the effects of Fe and Mn deficiencies and Mn toxicity on the protein profile of the xylem sap of tomato (Solanum lycopersicum), with the aim of elucidating plant response mechanisms to these nutritional stresses. Tomato was chosen as a model plant because the tomato genome has been published and this plant species has adequate root pressure and turgid stems that permit xylem sap sampling in sufficient amounts by de-topping. The high-throughput shotgun analysis has permitted to identify and quantitate a large number of low abundance proteins in the tomato xylem sap.
2017-11-30 | PXD007517 | Pride
Project description:Xylem sap of young cabbage plantlets was recovered from root pressure exudation and used as a growth medium for the vascular pathogen Xanthomonas campestris pv campestris, the causative agent of the black rot of Brassicaceae.
2014-01-04 | E-MTAB-2290 | biostudies-arrayexpress
Project description:Fusarium oxysporum f. sp. conlutinans (Foc) is a serious root-invading and xylem-colonizing fungus that causes yellowing in Brassica oleracea. To comprehensively understand the interaction between F. oxysporum and B. oleracea, composition of the xylem sap proteome of the non-infected and Foc-infected plants was investigated in both resistant and susceptible cultivars using liquid chromatography-tandem mass spectrometry (LC-MS/MS) after in-solution digestion of xylem sap proteins. Whole genome sequencing of Foc was carried out and generated a predicted Foc protein database. The predicted Foc protein database was then combined with the public B. oleracea and B. rapa protein databases downloaded from Uniprot and used for protein identification. About 200 plant proteins were identified in the xylem sap of susceptible and resistant plants. Comparison between the non-infected and Foc-infected samples revealed that Foc infection causes changes to the protein composition in B. oleracea xylem sap where repressed proteins accounted for a greater proportion than those of induced in both the susceptible and resistant reactions. The analysis on the proteins with concentration change >=2 fold indicated a large portion of up- and down-regulated proteins were those acting on carbohydrates. Proteins with leucine-rich repeats and legume lectin domains were mainly induced in both resistant and susceptible system, so was the case of thaumatins. Twenty-five Foc proteins were identified in the infected xylem sap and ten of them were cysteine-containing secreted small proteins that are good candidates for virulence and/or avirulence effectors. The findings of differential response of protein contents in the xylem sap between the non-infected and Foc-infected samples as well as the Foc candidate effectors secreted in xylem provide valuable insights into B. oleracea-Foc interactions.
2016-01-26 | PXD003378 | Pride
Project description:Total RNAs were extracted from internodes and roots of 18 day-after-germination soybean plants, and the expression levels of the genes that encode xylem sap associated peptides or proteins were analyzed.
2015-10-23 | E-MTAB-5052 | biostudies-arrayexpress
Project description:Xylem sap is the major transport route for nutrients from roots to shoots. In the present study, we investigated how variations in nitrogen (N) nutrition affected the metabolome and proteome of xylem sap and the growth of the xylem endophyte Brennaria salicis, and we also report transcriptional re-wiring of leaf defenses in poplar (Populus × canescens). We supplied poplars with high, intermediate or low concentrations of ammonium or nitrate. We identified 288 unique proteins in xylem sap. Approximately 85% of the xylem sap proteins were shared among ammonium- and nitrate-supplied plants. The number of proteins increased with increasing N supply but the major functional categories (catabolic processes, cell wall-related enzymes, defense) were unaffected. Ammonium nutrition caused higher abundances of amino acids and carbohydrates, whereas nitrate caused higher malate levels in xylem sap. Pipecolic acid and N-hydroxy-pipecolic acid increased, whereas salicylic acid and jasmonoyl-isoleucine decreased, with increasing N nutrition. Untargeted metabolome analyses revealed 2179 features in xylem sap, of which 863 were differentially affected by N treatments. We identified 124 metabolites, mainly from specialized metabolism of the groups of salicinoids, phenylpropanoids, phenolics, flavonoids, and benzoates. Their abundances increased with decreasing N, except coumarins. Brennaria salicis growth was reduced in nutrient-supplemented xylem sap of low- and high- NO<sub>3</sub> <sup>-</sup> -fed plants compared to that of NH<sub>4</sub> <sup>+</sup> -fed plants. The drastic changes in xylem sap composition caused massive changes in the transcriptional landscape of leaves and recruited defenses related to systemic acquired and induced systemic resistance. Our study uncovers unexpected complexity and variability of xylem composition with consequences for plant defenses.
2022-07-04 | MTBLS2305 | MetaboLights
Project description:Sudden death syndrome (SDS) is caused by the ascomycete fungus, Fusarium virguliforme. The pathogen secretes one or more toxins into the infected soybean roots to cause foliar SDS. This study investigated if the xylem sap of F. virguliforme-infected soybean plants contains secreted F. virguliforme-proteins, some of which could be involved in foliar SDS development.
2014-05-23 | PXD000873 | Pride