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PPDPF suppresses the development of hepatocellular carcinoma through TRIM21-mediated ubiquitination of RIPK1

ABSTRACT: Our study have shown that PPDPF promoted NF-κB activation through TRIM21-mediated K63-linked ubiquitination of RIPK1 in hepatocytes. Upon TNF-α stimulation, PPDPF enhances TRIM21/RIPK1 interaction and increases TRIM21-mediated K63-linked ubiquitination of RIPK1, which promotes NF-κB activation.To map the ubiquitination site of RIPK1 regulated by PPDPF, we performed MS analysis using control and RIPK1-overexpressing HEK293T cells with or without PPDPF overexpression

ORGANISM(S): Homo Sapiens

SUBMITTER: Jingjing Li

PROVIDER: PXD040301 | iProX | Tue Feb 21 00:00:00 GMT 2023

REPOSITORIES: iProX

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PPDPF suppresses the development of hepatocellular carcinoma through TRIM21-mediated ubiquitination of RIPK1.

Wang Yi-Kang YK Ma Ning N Xu Sheng S Huang Jing-Yi JY Ni Qian-Zhi QZ Cao Hui-Jun HJ Zheng Qian-Wen QW Zhu Bing B Xia Ji J Zhang Feng-Kun FK Ding Xu-Fen XF Qiu Xiao-Song XS Chen Tian-Wei TW Wang Kang K Chen Wei W Li Zhi-Gang ZG Cheng Shu-Qun SQ Xie Dong D Li Jing-Jing JJ

Cell reports 20230405 4

Pancreatic progenitor cell differentiation and proliferation factor (PPDPF) has been reported to play a role in tumorigenesis. However, its function in hepatocellular carcinoma (HCC) remains poorly understood. In this study, we report that PPDPF is significantly downregulated in HCC and the decreased PPDPF expression indicates poor prognosis. In the dimethylnitrosamine (DEN)-induced HCC mouse model, hepatocyte-specific depletion of Ppdpf promotes hepatocarcinogenesis, and reintroduction of PPDPF ...[more]

PMID: 37027301

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| PRJNA937032 | ENA

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