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KA120 negatively regulate immunity through inhibit MOS 4-associated-complex forming nuclear condensates

ABSTRACT: To understand how KA120 participates in plant immune regulation as a karyopherin-β, we sought to determine in vivo substrates of KA120. We leveraged an enzyme-catalyzed proximity-labeling strategy, in which we fused KA120 with TurboID. MAC3B could form nuclear condensates in ka120 mutant thus cause aberrant immune response. To investigate how the MAC function may be altered upon condensation and how that may contribute to immune activation, we profiled the composition of the MAC in the presence and absence of KA120.

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Yangnan Gu

PROVIDER: PXD040403 | iProX | Thu Feb 23 00:00:00 GMT 2023

REPOSITORIES: iProX

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Nuclear transport receptor KA120 regulates molecular condensation of MAC3 to coordinate plant immune activation.

Jia Min M Chen Xuanyi X Shi Xuetao X Fang Yiling Y Gu Yangnan Y

Cell host & microbe 20230914 10

The nucleocytoplasmic exchange is of fundamental importance to eukaryotic life and is mediated by karyopherins, a superfamily of nuclear transport receptors. However, the function and cargo spectrum of plant karyopherins are largely obscure. Here, we report proximity-labeling-based proteomic profiling of in vivo substrates of KA120, a karyopherin-β required for suppressing autoimmune induction in Arabidopsis. We identify multiple components of the MOS4-associated complex (MAC), a conserved splic ...[more]

PMID: 37714161

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