RNA MS2-tag based affinity method was used to purify 3' processing factors associated with intronic PAS processing

ABSTRACT: three copies of the MS2 hairpin were fused to the intronic PAS of the nr3c1 gene. To make a negative control RNA substrate, the core hexamer AAUAAA within PAS was mutated to AACAAA. Subsequently, the RNA substrate was incubated with HeLa NE, and the mRNA 3' processing complex was purified using an MS2 tag

ORGANISM(S): Homo Sapiens

SUBMITTER: Peng Xiang  

PROVIDER: PXD040405 | iProX | Mon Feb 27 00:00:00 GMT 2023

REPOSITORIES: iProX