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RNA MS2-tag based affinity method was used to purify 3' processing factors associated with intronic PAS processing

ABSTRACT: three copies of the MS2 hairpin were fused to the intronic PAS of the nr3c1 gene. To make a negative control RNA substrate, the core hexamer AAUAAA within PAS was mutated to AACAAA. Subsequently, the RNA substrate was incubated with HeLa NE, and the mRNA 3' processing complex was purified using an MS2 tag

ORGANISM(S): Homo Sapiens

SUBMITTER: Peng Xiang

PROVIDER: PXD040405 | iProX | Mon Feb 27 00:00:00 GMT 2023

REPOSITORIES: iProX

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The U1 antisense morpholino oligonucleotide (AMO) disrupts U1 snRNP structure to promote intronic PCPA modification of pre-mRNAs.

Feng Qiumin Q Lin Zejin Z Deng Yanhui Y Ran Yi Y Yu Rui R Xiang Andy Peng AP Ye Congting C Yao Chengguo C

The Journal of biological chemistry 20230522 7

Functional depletion of the U1 small nuclear ribonucleoprotein (snRNP) with a 25 nt U1 AMO (antisense morpholino oligonucleotide) may lead to intronic premature cleavage and polyadenylation of thousands of genes, a phenomenon known as U1 snRNP telescripting; however, the underlying mechanism remains elusive. In this study, we demonstrated that U1 AMO could disrupt U1 snRNP structure both in vitro and in vivo, thereby affecting the U1 snRNP-RNAP polymerase II interaction. By performing chromatin ...[more]

PMID: 37224962

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