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ITRAQ-based proteomic analysis provides novel insight into the postnatal testicular development of Hu sheep

ABSTRACT: The study was conducted to characterize the protein profiles at four key stages during postnatal testicular development, including infant (0-month-old, M0), puberty (3-month-old, M3), sexual maturity (6-month-old, M6) and body maturity (12-month-old, M12), and between the large- and small-testis groups at 6 months in Hu sheep. Consequently, 5252 proteins were identified using isobaric tags for relative and absolute quantification (iTRAQ) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods, and 465, 1261, 231 and 1080 differentially abundant proteins (DAPs) were found between M0_vs_M3, M3_vs_M6L, M6L_vs_M12, and M6L_vs_M6S, respectively. GO and KEGG analysis revealed that the majority of DAPs were involved in cellular process, metabolic process and immune system-related pathways. Furthermore, a protein-protein interaction (PPI) network was constructed using the 86 fertility-related DAPs, and five proteins with the highest degree were represented as hub proteins, including CTNNB1, ADAM2, ACR, HSPA2 and GRB2. This study provided new insights into the regulation mechanisms of postnatal testicular development and identified several potential biomarkers for selecting the high-fertility rams.

ORGANISM(S): Ovis Sp. Lf-2011

SUBMITTER: Xiangpeng Yue

PROVIDER: PXD040906 | iProX | Wed Jul 26 00:00:00 BST 2023

REPOSITORIES: iProX

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iTRAQ-based proteomic analysis provides novel insight into the postnatal testicular development of Hu sheep.

Pei Shengwei S Luo Jing J Weng Xiuxiu X Xu Yanli Y Bai Jingjing J Li Fadi F Li Wanhong W Yue Xiangpeng X

Journal of proteomics 20230628

Testicular development is an intricate and coordinated process in which thousands of proteins are involved in the regulation of somatic cells development and spermatogenesis. However, knowledge about the proteomic changes during postnatal testicular development in Hu sheep is still elusive. The study was conducted to characterize the protein profiles at four key stages during postnatal testicular development, including infant (0-month-old, M0), puberty (3-month-old, M3), sexual maturity (6-month ...[more]

PMID: 37390892

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ITRAQ-based proteomic analysis provides novel insight into the postnatal testicular development of Hu sheep

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iTRAQ-based proteomic analysis provides novel insight into the postnatal testicular development of Hu sheep.

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