Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cytoplasmic ZNF268a interactome upon SeV infection

ABSTRACT: Cytoplasmic fraction of Flag-ZNF268a-transfected HEK293T cells 6h post SeV infection were used for IP followed by LC-MS identification for ZNF268a interacting proteins

ORGANISM(S): Homo Sapiens

SUBMITTER: Mingxiong Guo

PROVIDER: PXD041079 | iProX | Fri Mar 24 00:00:00 GMT 2023

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Publications

TBK1-stabilized ZNF268a recruits SETD4 to methylate TBK1 for efficient interferon signaling.

Liu Yi Y Yin Wei W Zeng Xianhuang X Fan Jinhao J Liu Chaozhi C Gao Mingyu M Huang Zan Z Sun Guihong G Guo Mingxiong M

The Journal of biological chemistry 20231104 12

Sufficient activation of interferon signaling is critical for the host to fight against invading viruses, in which post-translational modifications have been demonstrated to play a pivotal role. Here, we demonstrate that the human KRAB-zinc finger protein ZNF268a is essential for virus-induced interferon signaling. We find that cytoplasmic ZNF268a is constantly degraded by lysosome and thus remains low expressed in resting cell cytoplasm. Upon viral infection, TBK1 interacts with cytosolic ZNF26 ...[more]

PMID: 37926288

Similar Datasets

CYP1B1-AS1 regulates CYP1B1 to promote Coxiella burnetii pathogenesis by inhibiting ROS and host cell death

Project description:ECL1_1173151: FLAG-IP, HEK293T transfected with pcDNA3.1 vector expressing N-term-3XFLAG tagged GFP ECL1_1173152: FLAG-IP, HEK293T transfected with pcDNA3.1 vector expressing N-term-3XFLAG tagged GFP ECL1_1173153: FLAG-IP, HEK293T transfected with pcDNA3.1 vector expressing N-term-3XFLAG tagged CYP1B1 ECL1_1173154: FLAG-IP, HEK293T transfected with pcDNA3.1 vector expressing N-term-3XFLAG tagged CYP1B1 ECL1_1173155: FLAG-IP, HEK293T transfected with pcDNA3.1 vector expressing N-term-3XFLAG tagged CYP1B1 and infected with C.burnetii NMII ECL1_1173156: FLAG-IP, HEK293T transfected with pcDNA3.1 vector expressing N-term-3XFLAG tagged CYP1B1 and infected with C.burnetii NMII

2024-12-23 | MSV000096725 | MassIVE

SIK2-dependent Phosphoproteome and Interactome upon Salmonella infection

Project description:HeLa cells transfected with non-targeting control- and anti-SIK2 siRNA were left uninfected or infected with Salmonella and lysed 30 min pi. Following tryptic-digest peptides were labeled using TMT-pro 16-plex and phosphopeptides enriched. The endogenously tagged SIK2-HA IP workflow. Hela wild type or endogenous SIK2-HA-tagged HeLa cells were left uninfected or infected with Salmonella and lysed 1 h post-infection, followed by HA-IP, Tryptic digest, TMT-labeling and LC-MS/MS analysis.

2021-05-06 | PXD021859 | Pride

miRNA arrays: Ago2 transfected vs. Mock, Ago2 IP vs. total RNA

Project description:miRNAs were enriched from HEK293T cells using the ambion FLASHPAGE fractionater after mock transfection, Ago2 transfection, and FLAG-Ago2 IP. miRNAs were labeled and hybridized: Ago2 transfected vs. mock transfection and Ago2 transfected vs. Ago2 IPs. Set of arrays that are part of repeated experiments Keywords: Biological Replicate

2008-04-08 | GSE11081 | GEO

miRNA arrays: Ago2 transfected vs. Mock, Ago2 IP vs. total RNA

2008-04-07 | E-GEOD-11081 | biostudies-arrayexpress

mRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected

Project description:As a core RISC component, Ago2 associates with miRNAs and target mRNAs. To identify these mRNAs, we ran lysate from HEK293T cells over a FLAG resin from 2 conditions: +FLAG-Ago2, +mock transfection. To identify mRNAs associated with specific miRNAs, we ran lysate from HEK293T cells over a FLAG resin from 2 conditions: +FLAG-Ago2 & miR-1, and +FLAG-Ago2 & miR-124. Set of arrays that are part of repeated experiments Compound Based Treatment: mock transfected Keywords: Biological Replicate

2008-04-08 | GSE11079 | GEO

Cellular transcriptome upon HSV-1 infection or DUX4 overexpression

Project description:We found that the germline transcription factor double homeobox 4 (DUX4) is upregulated upon infection with wild-type herpes simplex virus-1 (HSV-1). The goal of this experiment was to compare the cellular transcriptome of HEK293T cells that were infected with HSV-1 (KOS strain), or transfected with a plasmid encoding human DUX4.

2018-09-19 | GSE101435 | GEO

LIN-41 RIP-Seq

Project description:FLAG-GFP tagged LIN-41 was immunoprecipitated using FLAG dynabeads (Sigma) from lysates of young adult C.elegans. After washing steps, bound fraction (IP) was eluted from beads and associated RNAs were isolated by using the Pico pure RNA isloation kit (Invitrogen)

2018-04-25 | GSE106814 | GEO

Proteome and Modification of Stat3 and Malt1 dependent on Hectd3

Project description:We transfected HEK293T cells with HA-Ub, Flag-Stat3 or Flag-Malt1A, with or without Xpress-Hectd3. We performed Flag immunoprecipitation then conducted LC-MS/MS.

2019-01-16 | PXD011756 | Pride

mRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected

2008-04-08 | E-GEOD-11079 | biostudies-arrayexpress

The histone chaperone CAF-1 cooperates with the DNA methyltransferases to maintain Cd4 silencing in cytotoxic T cells

Project description:FLAG IP-MS of transfected HEK293 cells with FLAG tagged mouse Chaf1a constructs (T1, T10) or empty vector (EV).

2019-04-18 | PXD012669 | Pride