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Proteomic analysis of GM-CSF and M-CSF polarized bone marrow derived macrophages

ABSTRACT: Bone marrow cells were isolated, primed with M-CSF (M-BMDM) or GM-CSF (GM-BMDM) and cultured for 7 days. The proteomic difference between GM-BMDM and M-BMDM were analyzed to describe the phenotye and function of two types of macrophages.

ORGANISM(S): Mus Musculus

SUBMITTER: Jinbo Yang

PROVIDER: PXD041180 | iProX | Wed Mar 29 00:00:00 BST 2023

REPOSITORIES: iProX

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Integrated transcriptomic and metabolomic analysis reveals the metabolic programming of GM-CSF- and M-CSF- differentiated mouse macrophages.

Zhang Qianyue Q Song Qiaoling Q Liu Shan S Xu Yuting Y Gao Danling D Lu Peizhe P Liu Yuantao Y Zhao Guanghui G Wu Lihong L Zhao Chenyang C Yang Jinbo J

Frontiers in immunology 20230925

Macrophages play a critical role in the inflammatory response and tumor development. Macrophages are primarily divided into pro-inflammatory M1-like and anti-inflammatory M2-like macrophages based on their activation status and functions. <i>In vitro</i> macrophage models could be derived from mouse bone marrow cells stimulated with two types of differentiation factors: GM-CSF (GM-BMDMs) and M-CSF (M-BMDMs), to represent M1- and M2-like macrophages, respectively. Since macrophage differentiation ...[more]

PMID: 37818352

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RNAseq analysis of GM-CSF and M-CSF polarized bone marrow derived macrophages cultured on TC or noTC dishes

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Mouse bone marrow derived macrophages

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small RNAnext Generation Sequencing of bone marrow-derived macrophages treated in M-CSF withdrawal medium

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Genome wide transcriptional analysis of tissue macrophages and bone marrow derived macrophages (BMDMs)

2014-05-02 | E-GEOD-56682 | biostudies-arrayexpress

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2013-07-10 | E-GEOD-48629 | biostudies-arrayexpress

IL-4-primed bone marrow macrophages isolated from C57Bl6/J or Balb/cAnNCrl mice

Project description:To compare the expression profile of differentiated mouse bone marrow macrophages (BMM) in response to IL-4, we have employed whole genome microarray expression profiling. For this purpose, bone marrow cells were isolated from 8 to 12 weeks old C57BL6/J and Balb/cAnNCrl mice and cultured in the presence of the macrophage colony-stimulating factor (M-CSF). After seven days of culture, IL-4 was added for 4 and 18 hours. Keywords: Mice strain comparison; Gene expression profiling

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Comparative characterization of osteoclasts derived from murine bone marrow macrophages and RAW 264.7 cells using quantitative proteomics

Project description:Osteoclasts (OCs) are bone-resorbing cells differentiated from macrophage/monocyte precursors in response to M-CSF and RANKL. In vitro models are principally based on primary bone marrow macrophages, but RAW 264.7 cells are frequently used because they are widely available, easy to culture, and more amenable to genetic manipulation than primary cells. Increasing evidence, however, has shown that the vastly different origins of these two cell types may have important effects on cell behavior. In particular, M-CSF is prerequisite for the differentiation of BMMs, by promoting survival and proliferation and priming the cells for RANKL induction. RAW 264.7 cells readily form OCs in the presence of RANKL, but M-CSF is not required. Based on these key differences, we sought to understand their functional implications and how it might affect osteoclast differentiation and related signaling pathways. Using a robust and high-throughput proteomics strategy, we quantified the global protein changes in OCs derived from bone marrow macrophages and RAW 264.7 cells at 1, 3, and 5 days of differentiation. Correlation analysis of the proteomes demonstrated low concordance between the two cell types (R2 ≈ 0.13). Bioinformatics analysis indicate that RANKL-dependent signaling was intact in RAW 264.7 cells, but biological processes known to be dependent on M-CSF were significantly different; including cell cycle control, cytoskeletal organization, and apoptosis. RAW 264.7 cells exhibited constitutive activation of Erk and Akt that was dependent on the activity of Abelson tyrosine kinase, and the timing of Erk and Akt activation was significantly different between BMMs and RAW 264.7 cells. Our findings provide the first evidence for major differences between BMMs and RAW 264.7 cells, indicating that careful consideration is needed when using the RAW 264.7 cell line when studying M-CSF-dependent signaling and functions.

2018-11-27 | PXD009610 | Pride

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