Project title: Protein phosphorylation profiles in receptor-like kinase mutant tomato
Ontology highlight
ABSTRACT:
ORGANISM(S): Solanum Lycopersicum
SUBMITTER: 
Xiaojian Yin
PROVIDER: PXD041279 | iProX | Thu Mar 30 00:00:00 BST 2023
REPOSITORIES: iProX
Similar Datasets
Project description:To elucidate the molecular and metabolic changes in tomato plants that occur by the interaction of tomato roots and MBI600 elicitors, fifteen-day-old tomato seedlings were transferred to liquid MS (2 seedlings/2mL of medium in wells of 24-well-plates) and after 3 hours the medium was supplied with MBI600 CFCF to 1/5 v/v volume or with flg22 peptide (Eurogentec) to a final concentration of 1μΜ. Seedlings were collected in different time points after treatment (6 and 24h). Three independent experiments were performed. Each experiment included 2 biological replicates per treatment and each replicate was a pool of two seedlings. Sixteen genes were selected and their transcript levels were tested by Real-Time qPCR. A pathway analysis was performed and the complex signaling network that is implicated in MBI600-host interactions was mapped. The coordinated expression of genes that participate in biosynthetic pathways or are responsive to auxin, JA/ET, SA and redox signalling of biotic and abiotic stress responses, suggests that PGPR elicitors and metabolites activate (non-specifically) plant defense.
2018-12-11 | GSE123589 | GEO
Project description:Background: Plants attenuate their responses to a variety of bacterial and fungal pathogens, leading to higher incidences of pathogen infection at night. However, little is known about the molecular mechanism responsible for the light-induced defence response; transcriptome data would likely facilitate the elucidation of this mechanism. Results: In this study, we observed diurnal changes in tomato resistance to Pseudomonas syringae pv. tomato DC3000 (Pto DC3000), with the greatest susceptibility before midnight. Nightly light treatment, particularly red light treatment, significantly enhanced the resistance; this effect was correlated with increased salicylic acid (SA) accumulation and defence-related gene transcription. RNA-seq analysis revealed that red light induced a set of circadian rhythm-related genes involved in the phytochrome and SA-regulated resistance response. The biosynthesis and signalling pathways of multiple plant hormones (auxin, SA, jasmonate, and ethylene) were co-ordinately regulated following Pto DC3000 infection and red light, and the SA pathway was most significantly affected by red light and Pto DC3000 infection. This result indicates that SA-mediated signalling pathways are involved in red light-induced resistance to pathogens. Importantly, silencing of nonexpressor of pathogensis-related genes 1 (NPR1) partially compromised red light-induced resistance against Pto DC3000. Furthermore, sets of genes involved in redox homeostasis (respiratory burst oxidase homologue, RBOH; glutathione S-transferases, GSTs; glycosyltransferase, GTs), calcium (calmodulin, CAM; calmodulin-binding protein, CBP), and defence (polyphenol oxidase, PPO; nudix hydrolase1, NUDX1) as well as transcription factors (WRKY18, WRKY53, WRKY60, WRKY70) and cellulose synthase were differentially induced at the transcriptional level by red light in response to pathogen challenge. Conclusions: Taken together, our results suggest that there is a diurnal change in susceptibility to Pto DC3000 with greatest susceptibility in the evening. The red light induced-resistance to Pto DC3000 at night is associated with enhancement of the SA pathway, cellulose synthase, and reduced redox homeostasis. Four treatments including control, three biological replicates each treatment
2015-09-14 | E-GEOD-64087 | biostudies-arrayexpress
Project description:Plants have evolved mechanisms to perceive and face mechanical stress (MS) caused by physical forces, including compacted soils, winds, rain, pathogens, and interactions with animals and plants. Previous research indicated that applying mechanical treatment (MT) to Arabidopsis increases both xylem area and seed yield. To explore sustainable tomato production, we applied MT - combining stem bending, weighting, and particular touching - to 10-day-old seedlings, using a specific weight on the upper stem for 48 h. Two days after the treatment, we observed stem enlargement and increased the number of xylem vessels and area in MT plants. Additionally, we noticed earlier flowering, leading to increased tomato production. The transcriptome of MT-treated plants revealed significant changes in the expression of several essential genes involved in central metabolism, growth responses, and crucial phytohormone signalling. By studying different tomato mutants in the ethylene and auxin signalling pathways, we demonstrated that both hormones play essential roles in the plant responses to combined MT. Our findings suggest that combined MT generates a beneficial MS in tomato plants that induces plant morphoanatomical changes that promote early flowering and increased yield, providing a promising strategy for sustainable agriculture.
2025-07-01 | GSE296870 | GEO
Project description:To investigate the effect of ER stress on gene expressions in Tomato, RNA-seq was performed on 2 hours and 6 hours of tunicamycin applied samples.
2024-12-24 | GSE220689 | GEO
Project description:60 tomato RIL - Exploring genetic variation in the tomato (Solanum section Lycopersicon) clade by whole-genome sequencing
| PRJEB6659 | ENA
Project description:84 tomato accessions - Exploring genetic variation in the tomato (Solanum section Lycopersicon) clade by whole-genome sequencing
| PRJEB5235 | ENA
Project description:Background: Plants attenuate their responses to a variety of bacterial and fungal pathogens, leading to higher incidences of pathogen infection at night. However, little is known about the molecular mechanism responsible for the light-induced defence response; transcriptome data would likely facilitate the elucidation of this mechanism. Results: In this study, we observed diurnal changes in tomato resistance to Pseudomonas syringae pv. tomato DC3000 (Pto DC3000), with the greatest susceptibility before midnight. Nightly light treatment, particularly red light treatment, significantly enhanced the resistance; this effect was correlated with increased salicylic acid (SA) accumulation and defence-related gene transcription. RNA-seq analysis revealed that red light induced a set of circadian rhythm-related genes involved in the phytochrome and SA-regulated resistance response. The biosynthesis and signalling pathways of multiple plant hormones (auxin, SA, jasmonate, and ethylene) were co-ordinately regulated following Pto DC3000 infection and red light, and the SA pathway was most significantly affected by red light and Pto DC3000 infection. This result indicates that SA-mediated signalling pathways are involved in red light-induced resistance to pathogens. Importantly, silencing of nonexpressor of pathogensis-related genes 1 (NPR1) partially compromised red light-induced resistance against Pto DC3000. Furthermore, sets of genes involved in redox homeostasis (respiratory burst oxidase homologue, RBOH; glutathione S-transferases, GSTs; glycosyltransferase, GTs), calcium (calmodulin, CAM; calmodulin-binding protein, CBP), and defence (polyphenol oxidase, PPO; nudix hydrolase1, NUDX1) as well as transcription factors (WRKY18, WRKY53, WRKY60, WRKY70) and cellulose synthase were differentially induced at the transcriptional level by red light in response to pathogen challenge. Conclusions: Taken together, our results suggest that there is a diurnal change in susceptibility to Pto DC3000 with greatest susceptibility in the evening. The red light induced-resistance to Pto DC3000 at night is associated with enhancement of the SA pathway, cellulose synthase, and reduced redox homeostasis.
2015-09-14 | GSE64087 | GEO
Project description:RNA sequencing was performed to investigate the the response mechanism of tomato response to drought stress. C2H2-type zinc finger proteins are classic and extensively studied members of the zinc finger family. C2H2-type zinc finger proteins participate in plant growth, development and stress responses. In this study, 99 C2H2-type zinc finger protein genes were identified and classified into four groups, and many functionally related cis-elements were identified. Differential C2H2-ZFP gene expression and specific responses were analyzed under drought, cold, salt and pathogen stresses based on RNA-Seq data. Thirty-two C2H2 genes were identified in response to multiple stresses. Seven, 3, 5, and 8 genes were specifically expressed under drought, cold, salt and pathogenic stresses, respectively. Five glycometabolism and sphingolipid-related, pathways and the endocytosis pathway were enriched by KEGG analysis. The results of this study represent a foundation for further study of the function of C2H2-type zinc finger proteins and will provide us with genetic resources for stress tolerance breeding.
2020-04-13 | GSE148530 | GEO
Project description:We compared gene expression from 2C::tomato+/- ES cells from Kdm1a wt and mutant ES cultures 2C::tomato- samples 1, 5, 9 2C::tomato+ samples 2, 6, 10
2012-06-18 | GSE33763 | GEO
Project description:Proteins were extracted from tomato seedling (Heinz 1706) grown under 16-hour light/8-hour dark at 22 C for 4 days. Root consisted of ~3 cm from the tip and shoot consisted of cotyledons, meristems and ~1 cm hypocotyl. Proteins were then digested with either Trypsin/LysC or GluC, independently.
2019-01-25 | MSV000083363 | MassIVE