Proteomics

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Identification and quantitative proteomic analysis of a novel protein in GC-1 cell line and mouse testis.

ABSTRACT: We utilized a proteomic screening strategy to discover a novel protein encoded by an endogenous circRNA and we verified the existence of this protein by protein targeting quantitative experiments both in GC-1 cell line and in mouse testis. Simultaneously，we identified its interacting proteins using IP-MS and we performed protein quantitative analysis on mitochondria-related proteins and found that the absence of this protein would affect the function of mitochondria.

ORGANISM(S): Mus Musculus

SUBMITTER: Xuejiang Guo  

PROVIDER: PXD041424 | iProX | Sat Apr 08 00:00:00 BST 2023

REPOSITORIES: iProX

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Publications

A novel protein encoded by circRsrc1 regulates mitochondrial ribosome assembly and translation during spermatogenesis.

Zhang Shu S   Wang Chang C   Wang Yue Y   Zhang Hao H   Xu Chen C   Cheng Yiwei Y   Yuan Yan Y   Sha Jiahao J   Guo Xuejiang X   Cui Yiqiang Y  

BMC biology 20230424 1

<h4>Background</h4>Circular RNAs (circRNAs) are a large class of mammalian RNAs. Several protein products translated by circRNAs have been reported to be involved in the development of various tissues and systems; however, their physiological functions in male reproduction have yet not been explored.<h4>Results</h4>Here, we report an endogenous circRNA (circRsrc1) that encodes a novel 161-amino-acid protein which we named Rsrc1-161aa through circRNA sequencing coupled with mass spectrometry anal  ...[more]

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