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Presence of immune factors in freshwater mussel(Hyriopsis cumingii) entails autologous serum an essential component in the culture of mantle cells

ABSTRACT: Mussel cell culture is a challenging problem because serums play a vital biological role in cell culture as autologous donors and immune agents. In this project, Label-free proteomic analysis was carried out on fetal bovine serum and hyriopsis cumingii serum, and significant different proteins and hyriopsis cumingii specific proteins were analyzed. Most differentially expressed proteins are involved in immune responses, cell differentiation, and calcium ion binding. In addition, HSP, CALR, APOB, C3 and other immune factors were significantly different.

ORGANISM(S): Hyriopsis Cumingii

SUBMITTER: Wenjuan Li

PROVIDER: PXD041482 | iProX | Wed Apr 12 00:00:00 BST 2023

REPOSITORIES: iProX

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Presence of immune factors in freshwater mussel (Hyriopsis cumingii) entails autologous serum an essential component in the culture of mantle cells.

Bai Zhiyi Z Wang He H Li Xuenan X Shen Xiaoya X Chen Yige Y Fu Yuanshuai Y Li Wenjuan W

Frontiers in immunology 20230505

Mussel cell culture is a challenging problem and serum serves a crucial biological role in cell culture as an autologous supply and an immunizing agent. In this study, the biology (calcium ions, total protein, pH, and osmotic pressure) of fetal bovine serum (FBS) and Hyriopsis cumingii serum (HCS) was investigated, and the development of Hyriopsis cumingii (H. cumingii) mantle cells in HCS and FBS systems was examined. The results showed that total protein, calcium ions, and ...[more]

PMID: 37215128

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