Proteomics

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Organization, genomic targeting and assembly of three distinct SWI/SNF chromatin remodeling complexes in Arabidopsis


ABSTRACT: To comprehensively define the potential plant SWI/SNF sub-complexes and their subunits organization, we started by performing immuno-purification followed by mass spectrometry analysis (IP-MS) using stable transgenic Arabidopsis lines that expressed a green fluorescent protein (GFP)-tagged ATPase subunit, BRM (Li et al., 2016), SYD (Shu et al., 2021) or MINU2 (this study), driven by its native promoter in corresponding null mutant background.

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Chenlong Li  

PROVIDER: PXD041567 | iProX | Sat Apr 15 00:00:00 BST 2023

REPOSITORIES: iProX

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Publications

Organization, genomic targeting, and assembly of three distinct SWI/SNF chromatin remodeling complexes in Arabidopsis.

Fu Wei W   Yu Yaoguang Y   Shu Jie J   Yu Zewang Z   Zhong Yixiong Y   Zhu Tao T   Zhang Zhihao Z   Liang Zhenwei Z   Cui Yuhai Y   Chen Chen C   Li Chenlong C  

The Plant cell 20230601 7


Switch defective/sucrose nonfermentable (SWI/SNF) complexes are evolutionarily conserved multisubunit machines that play vital roles in chromatin architecture regulation for modulating gene expression via sliding or ejection of nucleosomes in eukaryotes. In plants, perturbations of SWI/SNF subunits often result in severe developmental disorders. However, the subunit composition, pathways of assembly, and genomic targeting of the plant SWI/SNF complexes are poorly understood. Here, we report the  ...[more]

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